Platelets are responsible for the accumulation of β-amyloid in blood clots inside and around blood vessels in mouse brain after thrombosis.
Platelets are responsible for the accumulation of β-amyloid in blood clots inside and around blood vessels in mouse brain after thrombosis.
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DOI：
10.1016/j.brainresbull.2016.11.008
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发表时间：
2017-01
影响因子：
3.8
通讯作者：
Inyushin MY
中科院分区：
文献类型：
--
作者：
Kucheryavykh LY;Dávila-Rodríguez J;Rivera-Aponte DE;Zueva LV;Washington AV;Sanabria P;Inyushin MY
血小板含有β -淀粉样蛋白前体蛋白（APP）以及可在激活后释放的β肽（Aβ）。在血栓形成过程中，血小板聚集成凝块并被激活。我们在小鼠体内使用荧光分析和电子显微镜来确定血小板在凝块中的浓度。我们用免疫染色法观察小鼠脑内光血栓形成后的Aβ。体内实验结果和电子显微镜都显示血小板在凝块中的浓度是在非凝块血液中的300-500倍。在对照组小鼠血栓形成后，Aβ免疫荧光在视觉皮层血管内和内嗅皮层毛细血管周围存在，而在血小板减少动物中不存在。血小板浓度的增加使得血栓形成过程中Aβ的释放增强，这表明如果阿尔茨海默病患者的大脑中发生频繁的微血栓事件，可能会出现阿尔茨海默病患者大脑中Aβ的额外来源。
Platelets contain beta-amyloid precursor protein (APP) as well as Aβ peptide (Aβ) that can be released upon activation. During thrombosis, platelets are concentrated in clots and activated. We used in vivo fluorescent analysis and electron microscopy in mice to determine to what degree platelets are concentrated in clots. We used immunostaining to visualize Aβ after photothrombosis in mouse brains. Both in vivo results and electron microscopy revealed that platelets were 300–500 times more concentrated in clots than in non-clotted blood. After thrombosis in control mice, but not in thrombocytopenic animals, Aβ immunofluorescence was present inside blood vessels in the visual cortex and around capillaries in the entorhinal cortex. The increased concentration of platelets allows enhanced release of Aβ during thrombosis, suggesting an additional source of Aβ in the brains of Alzheimer’s patients that may arise if frequent micro-thrombosis events occur in their brains.
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影响因子：
3.7
作者：
Gowert NS;Donner L;Chatterjee M;Eisele YS;Towhid ST;Münzer P;Walker B;Ogorek I;Borst O;Grandoch M;Schaller M;Fischer JW;Gawaz M;Weggen S;Lang F;Jucker M;Elvers M
通讯作者：
Elvers M
DOI：
10.1093/brain/awt308
发表时间：
2014-02
期刊：
Brain : a journal of neurology
影响因子：
--
作者：
Henderson SJ;Andersson C;Narwal R;Janson J;Goldschmidt TJ;Appelkvist P;Bogstedt A;Steffen AC;Haupts U;Tebbe J;Freskgård PO;Jermutus L;Burrell M;Fowler SB;Webster CI
通讯作者：
Webster CI
影响因子：
82.9
作者：
Deane, R;Yan, SD;Zlokovic, B
通讯作者：
Zlokovic, B
影响因子：
5.3
作者：
Collins, Jessica M.;King, Anna E.;Vickers, James C.
通讯作者：
Vickers, James C.
影响因子：
3.4
作者：
Kawahara, M;Arispe, N;Rojas, E
通讯作者：
Rojas, E