Overexpression of small glutamine‐rich TPR‐containing protein promotes apoptosis in 7721 cells
Overexpression of small glutamine‐rich TPR‐containing protein promotes apoptosis in 7721 cells
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DOI：
10.1016/j.febslet.2004.12.092
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发表时间：
2005-02
期刊：
影响因子：
3.5
通讯作者：
Hanzhou Wang;Hailian Shen;Yanlin Wang;Zejuan Li;Hongyan Yin;H. Zong;Jianhai Jiang;J. Gu
中科院分区：
文献类型：
--
作者：
Hanzhou Wang;Hailian Shen;Yanlin Wang;Zejuan Li;Hongyan Yin;H. Zong;Jianhai Jiang;J. Gu
富含谷氨酰胺的小分子TPR蛋白（SGT）是TPR基序家族的成员。然而，SGT的生物学功能尚不清楚。在本文中，我们报告，SGT在细胞凋亡信号中发挥作用。SGT的异位表达增强了诱导凋亡后的DNA片段和核断裂。在发生凋亡的7721细胞中也观察到SGT mRNA水平升高，敲低内源性SGT的表达有助于减少7721细胞的凋亡。缺失分析表明TPR结构域对SGT的促凋亡功能至关重要。此外，我们证明了当SGT在7721细胞中过表达时，PARP切割和细胞色素c释放在凋亡过程中增强。总的来说，我们的研究结果表明，SGT是一个新的促凋亡因子。
It is known that small glutamine-rich TPR-containing protein (SGT) is the member of TPR motif family. However, the biological functions of SGT remain unclear. In this paper, we report that SGT plays a role in apoptotic signaling. Ectopic expression of SGT enhances DNA fragment and nucleus breakage after the induction of apoptosis. Increasing mRNA level of SGT is also observed in 7721 cells undergoing apoptosis, knockdown the expression of endogenous SGT contributes to the decrease of apoptosis of 7721 cells. Deletion analysis reveals that TPR domain is critical to pro-apoptotic function of SGT. Furthermore, we demonstrated that the PARP cleavage and cytochrome c release are enhanced when SGT is overexpressed in 7721 cells during apoptosis. Collectively, our results indicate that SGT is a new pro-apoptotic factor.