Tumor-selective near-infrared photodynamic therapy with novel indocyanine green-loaded nanocarrier delays paralysis in rats with spinal metastasis
Tumor-selective near-infrared photodynamic therapy with novel indocyanine green-loaded nanocarrier delays paralysis in rats with spinal metastasis
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DOI：
10.1016/j.pdpdt.2013.03.002
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发表时间：
2013-12-01
影响因子：
3.3
通讯作者：
Sakane, Masataka
中科院分区：
文献类型：
--
作者：
Funayama, Toru;Tsukanishi, Toshinori;Sakane, Masataka
背景资料：虽然手术的最新进展提高了脊柱转移瘤患者的生活质量，但局部复发仍然相对常见。因此，迫切需要针对转移性脊柱癌的新手术治疗选择。我们之前描述了一种新型的纳米载体，载有吲哚菁绿色（ICG），ICG-乳糖体，其表现出肿瘤选择性，是一种潜在的近红外（NIR）荧光成像剂，用于诊断脊柱转移。本研究的目的是探讨肿瘤选择性光动力疗法（PDT）与ICG-乳糖体和NIR光照射在大鼠脊柱转移模型中的治疗效果。方法：21只Fischer 344大鼠，每只在L 6椎体中具有单个脊柱转移，被分为3个治疗组：光动力治疗加低浓度ICG-乳糖体注射组（6只）、光动力治疗加高浓度ICG-乳糖体注射组（7只）和不加ICG-乳糖体注射组（8只）。结果：与未注射ICG-lactosome组相比，注射ICG-lactosome的PDT组大鼠后肢瘫痪的恶化时间明显延迟。结论：改良的PDT治疗脊柱转移瘤是一种有效的局部治疗方法。(C)2013爱思唯尔有限公司版权所有。
Background: Although recent advances in surgery have improved the quality of life of patients with spinal metastasis, local recurrence is still relatively common. Therefore, there is an urgent need for new surgical treatment options for metastatic spinal cancer. We previously described a novel nanocarrier loaded with indocyanine green (ICG), ICG-lactosome which exhibits tumor selectivity and is a potential near-infrared (NIR) fluorescence imaging agent for the diagnosis of spinal metastasis. The purpose of this study was to investigate the therapeutic effects of tumor-selective photodynamic therapy (PDT) with ICG-lactosome and NIR light irradiation in a rat model of spinal metastasis.Methods: Twenty-one Fischer 344 rats each with a single spinal metastasis in the L6 vertebral body were divided into 3 treatment groups: PDT with a low-concentration ICG-lactosome injection (6 rats), PDT with high-concentration ICG-lactosome injection (7 rats), and a group without ICG-lactosome injection (8 rats). All the animals received local NIR light irradiation with a total energy of 5J (0.5W for 10 s).Results: Both the PDT groups injected with ICG-lactosome showed delayed deterioration of hind-limb paralysis compared with the group without ICG-lactosome.Conclusion: This modified PDT procedure could be an effective local treatment for spinal metastasis. (C) 2013 Elsevier B.V. All rights reserved.