INCREASED LEUKOCYTE PHOSPHOLIPASE-A2 ACTIVITY AND PLASMA LYSOPHOSPHATIDYLCHOLINE LEVELS IN ASTHMA AND RHINITIS AND THEIR RELATIONSHIP TO AIRWAY SENSITIVITY TO HISTAMINE
INCREASED LEUKOCYTE PHOSPHOLIPASE-A2 ACTIVITY AND PLASMA LYSOPHOSPHATIDYLCHOLINE LEVELS IN ASTHMA AND RHINITIS AND THEIR RELATIONSHIP TO AIRWAY SENSITIVITY TO HISTAMINE
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DOI：
10.1164/ajrccm/142.1.157
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发表时间：
1990-07-01
期刊：
影响因子：
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通讯作者：
AGRAWAL, KP
中科院分区：
文献类型：
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作者：
MEHTA, D;GUPTA, S;AGRAWAL, KP
本研究旨在测定正常人、哮喘和鼻炎患者的白细胞磷脂酶A2（PLA 2）活性和血浆溶血磷脂酰胆碱（LPC）水平，并探讨其与气道对组胺敏感性的关系。白细胞PLA 2活性和血浆LPC水平高度相关（rs = 0.90），并发现在两个疾病组，尤其是在哮喘患者显着升高。当患者和正常受试者一起检查时，PLA 2活性和LPC水平与组胺的对数剂量呈总体负相关，从而使比气道传导率（PD 35组胺）下降35%（分别为rs = 0.77和rs =-0.83）。白细胞PLA 2活性大于1.8U（30 ℃下nmol 14 C-AA释放/mg蛋白质/10分钟）的患者，C）或血浆LPC水平超过总磷脂的8.7%（主要是哮喘患者）显示出对组胺的高气道敏感性（PD 35组胺≤lmg/ml）。另一方面，白细胞PLA 2活性等于或小于1.96 U或血浆LPC水平等于或小于8.7%的患者的PD 35组胺值与正常受试者的PD 35组胺值在1.2至10 mg/ml的范围内重叠。血浆LPC水平的降低（可能反映气管支气管LPC水平，可能反映气管支气管LPC含量，如豚鼠所示）似乎是气道反应性恢复正常的重要步骤。
This study was carried out to determine leukocyte phospholipase A2 (PLA2) activity and plasma lysophosphatidylcholine (LPC) levels in normal subjects and in patients with asthma and rhinitis and to examine their relationship to airway sensitivity to histamine. Leukocyte PLA2 activity and plasma LPC levels were highly correlated (rs = 0.90), and were found significantly raised in both the disease groups, more so in the asthmatics. Both PLA2 activity and LPC levels showed overall negative correlations with the log dose of histamine producing a 35% fall in specific airway conductance (PD35 histamine) when the patients and the normal subjects were examined together (rs = 0.77 and rs =-0.83, respectively). The patients with leukocyte PLA2 activity more than 1.8 U (nmol 14C-AA released/mg protein/10 min at 30.degree. C) or plasma LPC levels more than 8.7% of total phospholipids (mostly asthmatics) showed high airway sensitivity to histamine (PD35 histamine .ltoreq. 1 mg/ml). On the other hand, the PD35 histamine values of patients with leukocyte PLA2 activity equal to or less than 1.96 U or plasma LPC levels equal to or less than 8.7% overlapped with those of normal subjects in the range of 1.2 to 10 mg/ml. Lowering of plasma LPC levels, which probably reflect tracheobronchial LPC levels, which probably reflect tracheobronchial LPC content as shown in guinea pigs, seems to be an essential step in the return of airway reactivity toward normal.