Multi-dimensional pulsed field gradient magic angle spinning NMR experiments on membranes
Multi-dimensional pulsed field gradient magic angle spinning NMR experiments on membranes
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DOI：
10.1002/mrc.1329
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发表时间：
2004-02-01
影响因子：
2
通讯作者：
Gawrisch, K
中科院分区：
文献类型：
--
作者：
Gaede, HC;Gawrisch, K
梯度技术在脂质膜研究中的优势在嵌入布洛芬的 1-棕榈酰-2-油酰-sn-甘油-3 磷酸胆碱 (POPC) 脂质体样品上得到了证明。溶液样品梯度 NMR 光谱的大多数技术可直接适用于经过魔角旋转 (MAS) 的膜样品。使用梯度增强同核和异核化学位移相关技术进行共振分配。梯度 NOESY 实验可深入了解脂质、布洛芬和水的位置和动态。梯度的应用不仅减少了实验时间，而且减少了多维光谱中的 t(1) 噪声。使用脉冲场梯度的扩散测量表征膜中脂质和药物分子的横向运动。提出了随机取向多层脂质体 MAS 扩散实验数据分析的理论框架。 John Wiley Sons, Ltd. 于 2004 年出版。
The benefits of gradient techniques in the study of lipid membranes are demonstrated on a sample of 1-palmitoyl-2-oleoyl-sn-glycero-3 phosphocholine (POPC) liposomes embedded with ibuprofen. Most techniques from gradient NMR spectroscopy on solution samples are directly applicable to membrane samples subjected to magic angle spinning (MAS). Gradient-enhanced homo- and heteronuclear chemical shift correlation techniques were used to make resonance assignments. Gradient NOESY experiments provide insight into the location and dynamics of lipids, ibuprofen and water. Application of gradients not only reduces experiment time but also the t(1) noise in the multi-dimensional spectra. Diffusion measurements with pulsed field gradients characterize lateral movements of lipid and drug molecules in membranes. The theoretical framework for data analysis of MAS diffusion experiments on randomly oriented multilamellar liposomes is presented. Published in 2004 by John Wiley Sons, Ltd.