Iron starvation regulates the type III secretion system in Bordetella bronchiseptica
Iron starvation regulates the type III secretion system in Bordetella bronchiseptica
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DOI：
10.1111/j.1348-0421.2012.00442.x
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发表时间：
2012-06-01
影响因子：
2.6
通讯作者：
Abe, Akio
中科院分区：
文献类型：
--
作者：
Kurushima, Jun;Kuwae, Asaomi;Abe, Akio
III型分泌系统（T3SS）在支气管脓毒杆菌发挥全部毒力中起关键作用。然而，对诱导T3SS基因表达的环境刺激知之甚少。本文报道铁饥饿是结核分枝杆菌T3SS基因表达的信号。结果发现，在缺铁条件下培养的支杆菌分泌的III型分泌蛋白比在缺铁条件下培养的细菌分泌的多。此外，研究证实，感染缺铁博德特拉菌可大大增强t3ss依赖性宿主细胞的细胞毒性和溶血活性。相反，在缺铁的博德特菌中，丝状血凝素的产生减少。因此，结核分枝杆菌控制毒力基因的表达，以应对铁饥饿。
The type III secretion system (T3SS) plays a key role in the exertion of full virulence by Bordetella bronchiseptica. However, little is known about the environmental stimuli that induce expression of T3SS genes. Here, it is reported that iron starvation is a signal for T3SS gene expression in B. bronchiseptica. It was found that, when B. bronchiseptica is cultured under iron-depleted conditions, secretion of type III secreted proteins is greater than that in bacteria grown under iron-replete conditions. Furthermore, it was confirmed that induction of T3SS-dependent host cell cytotoxicity and hemolytic activity is greatly enhanced by infection with iron-depleted Bordetella. In contrast, production of filamentous hemagglutinin is reduced in iron-depleted Bordetella. Thus, B. bronchiseptica controls the expression of virulence genes in response to iron starvation.