Effect of hyperbaric oxygen therapy on the intestinal ischemia reperfusion injury
Effect of hyperbaric oxygen therapy on the intestinal ischemia reperfusion injury
复制标题
DOI：
10.1590/s0102-86502011000600010
复制
发表时间：
2011-12-01
期刊：
影响因子：
--
通讯作者：
Féres, Omar
中科院分区：
文献类型：
--
作者：
Daniel, Rosemary Aparecida Furlan;Cardoso, Vinícius Kannen;Féres, Omar
目的：充分的组织氧合是愈合的关键。高压氧治疗(HBOT)在治疗缺血性病变方面具有潜在的临床应用价值，但其保护作用的确切性质目前尚不清楚。因此，我们研究了HBOT在肠I/R损伤模型中调节I/R损伤反应的潜在作用。方法：雄性Wistar大鼠采用手术诱导肠缺血再灌流。在肠缺血前和(或)缺血后给予HBOT。用四甲基偶氮唑盐比色法和血清丙二醛(MDA)测定检测肠组织细胞活性。结果：高压氧治疗可明显缩小缺血前后肠组织的病变范围。结论：高压氧治疗可通过诱导修复信号通路，抑制组织CD34细胞数量和血管内皮生长因子的表达，从而减轻缺血再灌注损伤对组织的损伤。
PURPOSE: Adequate tissue oxygenation is essential for healing. Hyperbaric oxygen therapy (HBOT) has potential clinical applications to treat ischemic pathologies, however the exact nature of any protective effects are unclear at present. We therefore investigated the potential role of HBOT in modulating the ischemia/reperfusion (I/R) injury response in intestinal model of I/R injury.METHODS: Male Wistar rats were subjected to surgery for the induction of intestinal ischemia followed by reperfusion. HBOT was provided before and/or after intestinal ischemia. Cell viability in the intestinal tissue was assessed using the MTT assay and by measuring serum malondealdehyde (MDA). Microvascular density and apoptosis were evaluated by immunohistochemistry.RESULTS: The results indicate that HBOT treatment pre- and post-ischemia reduces lesion size to the intestinal tissue. This treatment increases cell viability and reduces the activation of caspase-3, which is associated with increased number of tissue CD34 cells and enhanced VEGF expression.CONCLUSION: The hyperbaric oxygen therapy can limit tissue damage due to ischemia/reperfusion injury, by inducing reparative signaling pathways.