Activation of the isoflavonoid pathway in actinorhizal symbioses
Activation of the isoflavonoid pathway in actinorhizal symbioses
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DOI：
10.1071/fp11014
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发表时间：
2011-01-01
影响因子：
3
通讯作者：
Hocher, Valerie
中科院分区：
文献类型：
--
作者：
Auguy, Florence;Abdel-Lateif, Khalid;Hocher, Valerie
我们研究了黄酮类化合物在热带树木木麻黄glauca Sieb.来自Spreng。和放线菌Frankia。八个C。参与类黄酮生物合成的glauca基因：查耳酮合酶（CHS）、查耳酮异构酶（CHI）、黄酮还原酶（IFR）、类黄酮-3-羟化酶（F3 H）、类黄酮3 '-羟化酶（F3' H）、类黄酮3 '，5'羟化酶（F3 '5'H）、二氢黄酮醇4-还原酶（DFR）和黄酮醇合酶（FLS），从unigene数据库中鉴定，并通过定量实时PCR（qRT-PCR）在培养时间过程中监测基因表达模式。结果表明，FLS和F3 '5'H转录本在成熟根瘤中积累，而CHI和IFR转录本在接种Frankia后早期优先积累。在接种的植物和在有或无氮培养的对照植物中IFR和CHI表达的比较证实，IFR的早期表达与共生特异性相关。两者合计，这些数据表明，第一次，放线菌有牵连的放线菌。
We investigated the involvement of flavonoids in the actinorhizal nodulation process resulting from the interaction between the tropical tree Casuarina glauca Sieb. ex Spreng. and the actinomycete Frankia. Eight C. glauca genes involved in flavonoid biosynthesis: chalcone synthase (CHS), chalcone isomerase (CHI), isoflavone reductase (IFR), flavonoid-3-hydroxylase (F3H), flavonoid 3'-hydroxylase (F3'H), flavonoid 3',5' hydroxylase (F3'5'H), dihydroflavonol 4-reductase (DFR) and flavonol synthase (FLS), were identified from a unigene database and gene expression patterns were monitored by quantitative real-time PCR (qRT-PCR) during the nodulation time course. Results showed that FLS and F3'5'H transcripts accumulated in mature nodules whereas CHI and IFR transcripts accumulated preferentially early after inoculation with Frankia. Comparison of IFR and CHI expression in inoculated plants and in control plants cultivated with or without nitrogen confirmed that early expression of IFR is specifically linked to symbiosis. Taken together, these data suggest for the first time that isoflavonoids are implicated in actinorhizal nodulation.