Specific sequences of the env gene determine the host range of two XC-negative viruses of the Rauscher virus complex.
Specific sequences of the env gene determine the host range of two XC-negative viruses of the Rauscher virus complex.
复制标题
env 基因的特定序列决定了 Rauscher 病毒复合体的两种 XC 阴性病毒的宿主范围。
DOI：
10.1016/0042-6822(86)90470-8
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发表时间：
1986
期刊：
影响因子：
3.7
通讯作者：
Haas,M
中科院分区：
文献类型：
--
作者：
Vogt,M;Haggblom,C;Swift,S;Haas,M
已从 Rauscher 病毒 (RV) 复合体中分离出两种在标准 XC 测定（XC 阴性）中不产生 XC 斑块的病毒。这些病毒的宿主范围不同。其中一种是 R-MCF-1，具有双嗜性，因此会感染鼠类和非鼠类细胞。然而，与其他水貂细胞聚焦诱导 (MCF) 病毒不同，它不能感染 NIH 3T3 细胞。另一个，R-XC−，是亲生态的。它会感染小鼠细胞，包括 NIH 3T3 细胞，但不会感染水貂肺细胞。对杂交病毒（其中 R-MCF-1 和 R-XC-病毒基因组的同源区域交换）的分析表明，gp70 的 NH2 末端部分负责这些病毒的特定宿主范围。因此确定了R-XC-病毒的env基因的核苷酸序列，并与Rauscher和Friend病毒复合体的亲嗜性MLV和双嗜性MCF病毒的已知env序列进行比较。 R-XC-病毒被发现是一种重组病毒。它的env基因包含源自内源env基因的序列，该序列与MCF病毒的序列密切相关，但与任何先前描述的序列不同。 R-MCF-1和R-XC-病毒的特殊性质表明这两种病毒是由R-MLV和两个与已知MCF病毒不同的内源性序列之间重组而产生的。如果是这样，这表明小鼠基因组包含至少五个可以产生 MCF 样病毒的 env 序列。此外，由于 R-XC-病毒的宿主范围和干扰特性与之前描述的亲嗜性重组病毒非常相似 (4)，因此亲嗜性重组病毒可能是通过与 R-XC-病毒相同的内源序列重组而产生的。
Two viruses which do not give rise to XC plaques in the standard XC assay (XC-negative) have been isolated from the Rauscher virus (RV) complex. These viruses differ in their host range. One, R-MCF-1, is dualtropic and will therefore infect both murine and non-murine cells. However, unlike other mink cell focus-inducing (MCF) viruses, it cannot infect NIH 3T3 cells. The other, R-XC−, is ecotropic. It will infect murine cells, including NIH 3T3 cells, but does not infect mink lung cells. Analysis of hybrid viruses, in which homologous regions of the genomes of R-MCF-1 and R-XC−virus were exchanged, indicated that the NH2-terminal portion of the gp70 is responsible for the particular host ranges of these viruses. The nucleotide sequence of theenvgene of R-XC−virus was therefore determined and compared with the knownenvsequences of ecotropic MLVs and dualtropic MCF viruses of the Rauscher and Friend virus complexes. R-XC−virus was found to be a recombinant virus. Itsenvgene contained sequences derived from an endogenousenvgene which were closely related to those of the MCF viruses but differed from any previously described sequences. The particular properties of R-MCF-1 and R-XC−virus suggest that the two viruses arose by recombination between R-MLV and two endogenousenvsequences which differ from those of the known MCF viruses. If so, this suggests that the mouse genome contains at least fiveenvsequences which can give rise to MCF-like viruses. In addition, since the host range and interference properties of R-XC−virus are very similar to those of the previously described ecotropic recombinant viruses (4), it may be that the ecotropic recombinant viruses arose by recombination with the same endogenousenvsequences as did R-XC−virus.