Diagnosis of invasive septate mold infections - A correlation of microbiological culture and histologic or cytologic examination
Diagnosis of invasive septate mold infections - A correlation of microbiological culture and histologic or cytologic examination
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DOI：
10.1309/exbvyaupenbm285y
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发表时间：
2003-06-01
影响因子：
3.5
通讯作者：
Kontoyiannis, DP
中科院分区：
文献类型：
--
作者：
Tarrand, JJ;Lichterfeld, M;Kontoyiannis, DP
我们对2,891例来自尸检组织、手术或活检组织、支气管肺泡灌洗(BAL)或支气管冲洗(BW)的连续样本的微生物培养和组织病理学检查的结果进行了关联。23例尸检疑为侵袭性纵隔霉菌感染的尸检标本中，培养出霉菌12例(52%)。对于1,683个手术或活检样本，有30个样本存在侵袭性间隔霉菌感染的组织病理学证据，其中9个样本也通过培养产生霉菌(30%)；另外20个样本仅在培养中产生霉菌，可能代表培养污染。在1185份BAL和BW样本中，28份细胞学检查和培养发现霉菌，20份单独细胞学检查，68份单独培养。这些结果表明，培养和组织学细胞学检查的阳性符合率为23%，尽管这两种方法在96%的手术和活检组织以及BAL和BW样本中均为阴性。分离培养出的霉菌有烟曲霉19株、黄曲霉15株、土曲霉13株、黑曲霉7株、镰刀菌3株、尖生栅孢霉2株。从组织中分离出黄曲霉的频率明显高于从BAL和BW样本中分离到的黄曲霉。
We correlated results of microbiologic culture and histopathologic examination for 2,891 consecutive samples from autopsy tissue, surgical or biopsy tissue, and bronchoalveolar lavage (BAL) or bronchial washing (BW) specimens. For 23 autopsy cases with suspected invasive septate mold infections by histopathologic examination, culture yielded a mold in 12 cases (52%). For 1,683 surgical or biopsy samples, histopathologic evidence of invasive septate mold infection was present in 30 samples, 9 of which also grew mold by culture (30%); 20 additional samples grew mold in culture alone, possibly representing culture contamination. Of 1, 185 BAL and BW samples, mold was evident in 28 by cytologic examination and culture, 20 by cytologic examination alone, and 68 by culture alone. These results suggest a positive concordance for culture and histologic-cytologic examination of 23%, although both methods were negative in 96% of surgical and biopsy tissue and BAL and BW samples. The septate molds cultured from these samples were Aspergillus fumigatus (19), Aspergillus flavus (15), Aspergillus terreus (13), Aspergillus niger (7), Fusarium species (3), and Scedosporium apiospermum (2). A flavus was isolated significantly more frequently from tissue than from BAL and BW samples.