An Electrohydrodynamic Bioprinter for Alginate Hydrogels Containing Living Cells
An Electrohydrodynamic Bioprinter for Alginate Hydrogels Containing Living Cells
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DOI：
10.1089/ten.tec.2014.0149
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发表时间：
2015-02-01
影响因子：
3
通讯作者：
Migliaresi, Claudio
中科院分区：
文献类型：
--
作者：
Gasperini, Luca;Maniglio, Devid;Migliaresi, Claudio
在这项工作中，我们提出了一种生物打印技术，利用电流体动力学过程，以获得含有细胞的液体藻酸盐珠射流。打印机用于按照自下而上的方法逐块微制造水凝胶。藻酸盐珠粒构成微加工结构的构建块。将微珠放置在由富含钙的明胶制成的目标基质上的预定位置，在那里它们在接触时交联，而不需要进一步的后处理。打印的样品可以在生理温度下容易地从基底上移除。三维打印是通过沉积多层水凝胶来实现的。我们已经研究了影响过程的参数，印刷过程与细胞的兼容性，以及印刷后的存活率。
In this work we present a bioprinting technique that exploits the electrohydrodynamic process to obtain a jet of liquid alginate beads containing cells. A printer is used to microfabricate hydrogels block by block following a bottom-up approach. Alginate beads constitute the building blocks of the microfabricated structures. The beads are placed at predefined position on a target substrate made of calcium-enriched gelatin, where they crosslink upon contact without the need of further postprocessing. The printed sample can be easily removed from the substrate at physiological temperature. Three-dimensional printing is accomplished by the deposition of multiple layers of hydrogel. We have investigated the parameters influencing the process, the compatibility of the printing procedure with cells, and their survival after printing.