Enhanced plant regeneration from microspore-derived embryos of Brassica napus by chilling, partial desiccation and age selection
Enhanced plant regeneration from microspore-derived embryos of Brassica napus by chilling, partial desiccation and age selection
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通过冷冻、部分干燥和年龄选择增强甘蓝型油菜小孢子衍生胚的植物再生
DOI：
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发表时间：
1990
影响因子：
3
通讯作者：
W. Beversdorf
中科院分区：
文献类型：
--
作者：
L. Kott;W. Beversdorf
甘蓝型油菜“Topas”的小孢子来源的胚在萌发前暴露于一段时间的低温（4°C下9-12天）或部分干燥（快速或缓慢空气干燥）时容易诱导萌发。一般来说，35天大的胚胎与更年轻或更老的胚胎相比具有最高的发芽率。在特定的温度、干燥和年龄条件下，胚群体的萌发率可达90%以上。与胚胎发生B的比较。甘蓝型油菜冬种系F346。
Germination was readily induced in recalcitrant microspore-derived embryos of Brassica napus ‘Topas’ when they were exposed to a period of chilling (9–12 days at 4°C) or partial desiccation (rapid or slow air drying) prior to germination. In general, embryos thirty-five days old had the highest germination rates as compared to younger or older ones. Populations of embryos were induced to germinate at a rate of over 90% under specific temperature, desiccation and age conditions. Comparisons to an embryogenic B. napus winter line, F346, are made.