Gene structure and in situ transcript localization of pathogenesis-related protein 1 in parsley

Gene structure and in situ transcript localization of pathogenesis-related protein 1 in parsley
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DOI:
10.1007/bf00333403
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发表时间:
1988-07
期刊:
Molecular and General Genetics MGG
影响因子:
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通讯作者:
I. Somssich;E. Schmelzer;P. Kawalleck;K. Hahlbrock
I. Somssich;E. Schmelzer;P. Kawalleck;K. Hahlbrock
中科院分区:
其他
文献类型:
--
作者:
I. Somssich;E. Schmelzer;P. Kawalleck;K. Hahlbrock

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我们已经分析了三个几乎全长cDNA互补的mRNA编码两个PR 1(发病机制相关,1类)蛋白在欧芹(岩芹)。此外,一个选定的基因组克隆含有PcPR 1 -1基因进行了详细研究。并对该基因5′侧翼区的结构组成和可能的调控元件进行了分析。在真菌感染的欧芹叶组织中的原位RNA杂交表明,感染部位周围的快速和大量的PR 1 mRNA积累。
We have analysed three nearly full-length cDNAs complementary to mRNAs encoding two PR1 (pathogenesis-related, class 1) proteins in parsley (Petroselinum crispum). Furthermore, one selected genomic clone containing the PcPR1-1 gene was investigated in detail. The structural organization and possible regulatory elements in the 5′ flanking region of this gene are presented. In situ RNA hybridization in fungus-infected parsley leaf tissue demonstrated rapid and massive PR1 mRNA accumulation around infection sites.