Red-shifted optogenetic excitation:: a tool for fast neural control derived from Volvox carteri
Red-shifted optogenetic excitation:: a tool for fast neural control derived from Volvox carteri
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DOI：
10.1038/nn.2120
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发表时间：
2008-06-01
影响因子：
25
通讯作者：
Deisseroth, Karl
中科院分区：
文献类型：
--
作者：
Zhang, Feng;Prigge, Matthias;Deisseroth, Karl
两种微生物视紫红质-2(ChR2)和卤视紫红质(NpHR)这两种微生物视紫红质工具被引入神经科学，引起了人们对快速、多模式、细胞类型特定的神经回路控制的兴趣。在这里，我们描述了来自Volvox carteri的阳离子传导通道视紫红质(VChR1)，它可以驱动589 nm的尖峰，与ChR2相比，激发最大红移类似于70 nm。这些结果证明了黄光的快速光刺激作用，从而定义了微生物视紫红质蛋白的第三种不同功能。
The introduction of two microbial opsin- based tools, channelrhodopsin-2 (ChR2) and halorhodopsin (NpHR), to neuroscience has generated interest in fast, multimodal, cell type-specific neural circuit control. Here we describe a cation-conducting channelrhodopsin (VChR1) from Volvox carteri that can drive spiking at 589 nm, with excitation maximum red-shifted similar to 70 nm compared with ChR2. These results demonstrate fast photostimulation with yellow light, thereby defining a functionally distinct third category of microbial rhodopsin proteins.