Functional Characterization of a Novel Glycosyltransferase (UGT73CD1) from Iris tectorum Maxim. for the Substrate promiscuity
Functional Characterization of a Novel Glycosyltransferase (UGT73CD1) from Iris tectorum Maxim. for the Substrate promiscuity
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来自 Iris tectorum Maxim 的新型糖基转移酶 (UGT73CD1) 的功能表征。
DOI：
10.1007/s12033-021-00364-1
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发表时间：
2021-07-01
影响因子：
2.6
通讯作者：
Ji, Aijia
中科院分区：
文献类型：
--
作者：
Huang, Jia;Li, Jing;Ji, Aijia
糖基类黄酮是一类具有多种药理活性的天然产物，许多植物中的糖基转移酶参与了这些化合物的生物合成。然而，从著名的园艺和药用植物鸢尾（Iris tectorum Maxim）中还没有鉴定出相应的糖基转移酶。在此，UGT 73 CD 1，一种新的糖基转移酶，从I.顶盖基于转录组分析和功能鉴定。系统发育分析表明，UGT 73 CD 1属于类黄酮7-OH OGTs分支。生化分析表明，UGT 73 CD 1能够在7-OH处糖基化鸢尾苷元以产生鸢尾苷，因此被指定为7-O-糖基转移酶。此外，它还对12种结构不同的黄酮类化合物支架和3，4-二氯苯胺具有强大的催化混杂性，导致形成O-和N-糖苷。这项工作将为药物发现提供结构多样的黄酮苷的有效生物合成的见解。
Glycosylflavonoids are a class of natural products with multiple pharmacological activities and a lot of glycosyltransferases from various plant species have been reported that they were involved in the biosynthesis of these phytochemicals. However, no corresponding glycosyltransferase has been identified from the famous horticultural and medicinal plant Iris tectorum Maxim. Here, UGT73CD1, a novel glycosyltransferase, was identified from I. tectorum. based on transcriptome analysis and functional identification. Phylogenetic analysis revealed that UGT73CD1 grouped into the clade of flavonoid 7-OH OGTs. Biochemical analysis showed that UGT73CD1 was able to glycosylate tectorigenin at 7-OH to produce tectoridin, and thus assigned as a 7-O-glycosyltransferase. In addition, it also possessed robust catalytic promiscuity toward 12 structurally diverse flavonoid scaffolds and 3, 4-dichloroaniline, resulting in forming O- and N-glycosides. This work will provide insights into efficient biosynthesis of structurally diverse flavonoid glycosides for drug discovery.