T cells in G1 provide a memory-like response to secondary stimulation
T cells in G1 provide a memory-like response to secondary stimulation
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DOI：
10.4049/jimmunol.174.7.4010
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发表时间：
2005-04-01
影响因子：
4.4
通讯作者：
Ashwell, JD
中科院分区：
文献类型：
--
作者：
Munitic, I;Ryan, PE;Ashwell, JD
初始 T 细胞增殖的承诺是 TCR 和辅助受体介导的刺激强度和持续时间的函数。据报道，2-20 小时的刺激范围是体外必要的。 T细胞是否真的在生理条件下经历如此长时间的不间断刺激仍存在争议。在这里，我们要问的是，增殖的承诺是否需要持续刺激，或者 T 细胞是否可以整合间歇性刺激。 T 细胞被连续刺激或间隔一段时间刺激两个短期（阈值下）时期（5-7 小时）。初始淋巴结 T 细胞能够整合中断的刺激，即使休息时间长达 2 天。此外，当短期刺激的 T 细胞按密度分离时，观察到三个群体：低密度原始细胞、中密度 G, 细胞和高密度 G,, 细胞。低密度细胞在没有进一步刺激的情况下进行分裂，而G和G细胞保持不分裂。然而，经过一段时间的休息后，第二次阈下刺激导致 G，而不是 G，部分快速进入细胞周期。我们得出的结论是，细胞周期 G 期的非循环 T 细胞在较长时间内保持准备状态，并且可能代表能够快速响应抗原挑战的记忆样效应器群体。
The commitment of naive T cells to proliferate is a function of the strength and duration of stimuli mediated by the TCR and coreceptors. Ranges of 2-20 h of stimulation have been reported as necessary in vitro. Whether T cells actually experience uninterrupted stimulation for such long periods under physiological conditions is controversial. Here we ask whether commitment to proliferate requires continuous stimulation, or can T cells integrate intermittent periods of stimulation. T cells were stimulated for two short-term (subthreshold) periods (5-7 h) either sequentially or separated by an interval of rest. Naive lymph node T cells were able to integrate interrupted stimulation, even when the duration of rest was as long as 2 days. Furthermore, when short-term-stimulated T cells were separated by density, three populations were observed: low density blasts, intermediate density G, cells, and high density G,, cells. Low density cells progressed to division without further stimulation, whereas G, and G, cells remained undivided. However, after a period of rest, a second subthreshold stimulation caused the G, but not the G,, fraction to quickly proceed through the cell cycle. We conclude that noncycling T cells in the G, phase of the cell cycle remain in a state of readiness for prolonged periods of time, and may represent a population of memory-like effectors capable of responding rapidly to antigenic challenge.