Occurrence of occult hepatitis B virus infection associated with envelope protein mutations according to anti-HBs carriage in blood donors
Occurrence of occult hepatitis B virus infection associated with envelope protein mutations according to anti-HBs carriage in blood donors
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根据献血者抗-HBs携带情况，隐匿性乙型肝炎病毒感染的发生与包膜蛋白突变相关
DOI：
10.1016/j.ijid.2019.12.026
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发表时间：
2020-03-01
影响因子：
8.4
通讯作者：
Li, Tingting
中科院分区：
文献类型：
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作者：
Wang, Jiawen;Zhang, Panli;Li, Tingting
目的：隐匿性乙型肝炎病毒感染（OBI）具有乙型肝炎病毒（HBV）传播和肝细胞癌的风险。由于之前的研究样本量有限，尚未充分探讨与疫苗接种引起的乙型肝炎表面抗体（抗-HBs）相关的基因型B和C（OBIB和OBIC）突变或对乙型肝炎病毒的有限宿主免疫反应相关的OBIB或OBIC菌株的发生情况。 方法：在本研究中，从根据抗-HBs携带分层的99名献血者中获得与包膜蛋白（前S/S）氨基酸取代相关的OBIB或OBIC菌株的发生情况结果：根据每个基因型中抗-HBs的存在情况，抗-HBs（类似）OBIB特异性取代位点的数量和频率高于抗-HBs（+）OBIB菌株特异性取代位点的数量和频率（67 vs 31；117 vs 41），但在OBIC菌株中发现相反的模式（3 vs 24；3 vs 26）。突变pre-s(1)T68I和sQ129R/L在15-25%的抗HBs(+)OBIB携带者中独特发现，突变pre-s(1)A54E优先在抗HBs(+)OBIC中发现，而17个取代优先在11-38%的抗HBs(类似)OBIB菌株中发现。在主要亲水区（MHR）区域，OBIB中的sS167突变、OBIC中的sT118突变以及两种基因型中的sA166可能是免疫诱导的逃逸突变位点。结论：OBI前S/S中的多个突变似乎与携带者抗HBs压力有关，这可能是OBI携带者抗HBs选择下病毒潜在再激活的危险因素。 (C) 2019 作者。由爱思唯尔有限公司代表国际传染病学会出版。
Objectives: Occult hepatitis B virus infection (OBI) carries a risk of hepatitis B virus (HBV) transmission and hepatocellular carcinoma. As previous studies have had a limited sample size, the characteristics of OBI with genotype B and C (OBIB and OBIC) mutations relating to hepatitis B surface antibody (anti-HBs) elicited by vaccination or a limited host immune response to HBV have not been fully explored.Methods: In this study, the occurrence of OBIB or OBIC strains associated with envelope protein (pre-S/S) amino acid substitutions obtained from 99 blood donors stratified according to anti-HBs carriage were characterized extensively.Results: According to the presence of anti-HBs within each genotype, the number and frequency of substitution sites specific for anti-HBs(similar to) OBIB were higher than those specific for anti-HBs(+) OBIB strains (67 vs 31; 117 vs 41), but the reverse pattern was found in OBIC strains (3 vs 24; 3 vs 26). Mutations pre-s(1)T68I and sQ129R/L were found uniquely in 15-25% of anti-HBs(+) OBIB carriers and mutation pre-s(1)A54E was found preferentially in anti-HBs(+) OBIC, while 17 substitutions were found preferentially in 11-38% of anti-HBs(similar to) OBIB strains. In the major hydrophilic region (MHR) region, mutations sS167 in OBIB, sT118 in OBIC, and sA166 in both genotypes were possibly immune-induced escape mutation sites.Conclusions: Several mutations in pre-S/S of OBI appeared to be associated with carrier anti-HBs pressure, which might be risk factors for potential reactivation of viruses under anti-HBs selection in OBI carriers. (C) 2019 The Author(s). Published by Elsevier Ltd on behalf of International Society for Infectious Diseases.