Prevention of phosphate-induced progression of uremia in rats by 3-phosphocitric acid.
Prevention of phosphate-induced progression of uremia in rats by 3-phosphocitric acid.
复制标题
3-磷酸柠檬酸预防磷酸盐诱导的大鼠尿毒症进展。
DOI：
10.1038/ki.1982.129
复制
发表时间：
1982
影响因子：
19.6
通讯作者：
Hermann,JA
中科院分区：
文献类型：
--
作者：
Gimenez,L;Walker,WG;Tew,WP;Hermann,JA
方法建立肾功能不全的残肾模型。32只体重在250和300 g之间的雄性Sprague-Dawley大鼠在戊巴比妥-乙醚麻醉下通过右腹切口进行右肾切除术。在右腹切口闭合后，通过结扎并连续切除上、下极实现左肾部分切除术，保留中间三分之一完整作为功能节段。取出胶囊后，对右肾进行称重，估计肾脏总质量的重量是右肾重量的两倍[141.分别对左肾的上段和下段进行称重，并使用以下公式估计剩余残余物的重量：2（右肾重量）-右肾重量+左肾上、下三分之一的总重量。所有动物术后恢复2周，然后随机分为三组：（1）对照组（5只动物）。这些动物接受由8%脂肪、20%蛋白质和72%碳水化合物组成的合成饲料，每100 g饲料含有17.7 mmol钙和18.8 mmol磷，加上1 mg维生素A和200 IU维生素D [21]。在该方案中，基于该对照组中每只大鼠每天19 g食物的平均食物摄入量，计算平均每日磷酸盐摄入量为3.6 mmol/只大鼠每天。(2)磷酸盐喂养组（12只动物）。这些动物接受与对照组相同的饲料，通过添加磷酸氢二钠调整至含71 mmol磷酸盐/100 g饲料。每只大鼠每天平均食物摄入量为16.9 g，估计磷摄入量为12 mmol/24 hr。(3)磷酸柠檬酸盐处理（15只动物）。这些动物的治疗与磷酸盐喂养组相同，但也接受50皮摩尔的3-磷酸柠檬酸作为钠盐每天腹膜内给药。这些动物的食物摄入量平均为19.7 g/天，磷摄入量为14 mmol/24 hr。每周对所有动物称重，并测定24 hr期间的总食物和水摄入量。在手术前、手术后立即和实验开始后大约每两周采集一次血液用于肌酐测定。
Methods Production of remnant kidney model of renal insufficiency. Thirty-two male Sprague-Dawley rats weighing between 250 and 300 g were subjected to right nephrectomy through a right flank incision under nembutal-ether anesthesia. Following closure of the right flank incision, a partial left nephrectomy was achieved by ligating and removing successively the upper and lower poles, leaving the middle third intact as the functioning segment. After removal of the capsule, the right kidney was weighed, and the weight of the total renal mass was estimated to be twice the weight of the right kidney [141. The upper and lower segments of the left kidney were weighed separately, and weight of the remaining remnant was estimated using the following formula: 2 (weight right kidney)—weight right kidney+ total weights of upper and lower thirds of left kidney. All animals were allowed 2 weeks to recover from surgery, and then they were randomized into three groups:(1) Control group (five animals). These animals received a synthetic diet consisting of 8% fat, 20% protein and 72% carbohydrate and containing 17.7 mmoles of calcium and 18.8 mmoles of phosphorus plus 1 mg of vitamin A and 200 IU of vitamin D per 100 g of diet [21]. On this regimen the average daily phosphate intake was calculated as 3.6 mmoles per rat per day based upon an average food intake of 19 g of food per rat per day in this control group.(2) Phosphate-fed group (12 animals). These animals re-ceived the same diet as the control group, adjusted to contain 71 mmoles of phosphate/100 g diet by addition of dibasic sodium phosphate. Food intake averaged 16.9 g per rat per day, providing an estimate of phosphorus intake of 12 mmoles/24 hr.(3) Phosphocitrate-treated (15 animals). These animals were treated identically to the phosphate-fed group but also received 50 pmoles of 3-phosphocitric acid given intraperitoneally daily as the sodium salt. Food intake averaged 19.7 g per day in these animals, providing a phosphorus intake of 14 mmoles/24 hr. Each week all animals were weighed and total food and water intake determined for one 24-hr period. Blood was taken for creatinine determination before surgery, immediately after surgery and at approximately biweekly intervals after the experiment was begun.