Determination of antioxidative potential of lichen Usnea ghattensis in vitro
Determination of antioxidative potential of lichen Usnea ghattensis in vitro
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DOI：
10.1016/j.lwt.2004.11.007
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发表时间：
2006-01-01
影响因子：
6
通讯作者：
Makhija, U
中科院分区：
文献类型：
--
作者：
Behera, BC;Verma, N;Makhija, U
研究松萝提取物的抗氧化活性。研究了其抗氧化性、还原力、清除超氧阴离子自由基和自由基的能力。抗氧化活性随提取物添加量的增加而增加（2- 20 mg/ml）。20 mg/ml的提取物对脂质过氧化的抑制率可达73.3%，相同浓度的α-生育酚对脂质过氧化的抑制率可达39.2%。与对照组比较，有显著性差异（r（2）= 0.876，P < 0.01）。而PMS/NADH-NBT法测定其对超氧阴离子的清除作用不明显。与抗氧化活性一样，提取物的还原力和自由基清除活性依赖于浓度，并随着样品量的增加而增加。结果表明，U.发现ghattensis提取物的抗氧化性大于BHA和BHT。本研究结果表明，乌藨子是一种潜在的天然抗氧化剂来源。(c)2004年，瑞士食品科学与技术协会。由爱思唯尔有限公司出版。保留所有权利。
The study was aimed at evaluating the antioxidant activities of extract of Usnea ghattensis. The antioxidant activity, reducing power, superoxide anion radical scavenging and free radical scavenging activities were studied. The antioxidant activity increased with the increasing amount of extracts (2-20mg/ml) added to the linoleic acid emulsion. Lipid peroxidation upto 73.3% was inhibited by the extract of 20 mg/ml and 39.2% by alpha-tocopherol at the same concentration. These effects were statistically significant (r(2) = 0.876, P < 0.01) when compared with control. However, the extract had no significant effect on superoxide anion scavenging by the PMS/NADH-NBT method. Like antioxidant activity, the reducing power and free radical scavenging activity of extract depends on concentration and increasing with increased amount of sample. The reducing power and DPPH radical-scavenging activity of U. ghattensis extract were found greater than the BHA and BHT. The results obtained in the present study indicate that U ghattensis is a potential source of natural antioxidant. (c) 2004 Swiss Society of Food Science and Technology. Published by Elsevier Ltd. All rights reserved.