Neuronal and synaptic protein lifetimes
Neuronal and synaptic protein lifetimes
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DOI：
10.1016/j.conb.2018.12.007
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发表时间：
2019-08-01
影响因子：
5.7
通讯作者：
Ziv, Noam E.
中科院分区：
文献类型：
--
作者：
Cohen, Laurie D.;Ziv, Noam E.
神经元蛋白质稳态受到神经元非凡结构、它们形成的大量突触以及精确保存单个突触功能的需要的独特挑战。蛋白质寿命的定量信息可以为如何应对这些挑战提供线索。蛋白质组学和质谱学的进步，现在可以对数千种蛋白质进行全面的寿命估计，这表明神经元和突触蛋白质的寿命异常长，对于某些蛋白质家族，半衰期通常从几天到几周，甚至几个月甚至更长。体内半衰期比细胞培养物中的半衰期长几倍，倾向于聚集属于多分子复合物的蛋白质，受发育阶段影响，并可能受环境条件和活性水平影响。
Neuronal proteostasis is uniquely challenged by the extraordinary architecture of neurons, the vast number of synapses they form, and the need to precisely preserve function at individual synapses. Quantitative information on protein lifetimes can provide clues as to how these challenges are met. Advances in proteomics and mass spectrometry, which now enable comprehensive lifetime estimations for thousands of proteins, suggest that neuronal and synaptic protein lifetimes are unusually long, with half-lives typically ranging from days to weeks, even months and beyond for certain protein families. Half-lives in vivo are several-fold longer than those in cell culture, tend to cluster for proteins belonging to multimolecular complexes, are affected by developmental stage, and possibly by environmental conditions and activity levels.