Ectopic expression of an apple cytochrome P450 gene MdCYPM1 negatively regulates plant photomorphogenesis and stress response in Arabidopsis
Ectopic expression of an apple cytochrome P450 gene MdCYPM1 negatively regulates plant photomorphogenesis and stress response in Arabidopsis
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苹果细胞色素P450基因MdCYPM1的异位表达负调控拟南芥植物光形态建成和胁迫反应
DOI：
10.1016/j.bbrc.2017.01.026
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发表时间：
2017-01-29
影响因子：
3.1
通讯作者：
Hao, Yu-Jin
中科院分区：
文献类型：
--
作者：
An, Jian-Ping;Li, Rui;Hao, Yu-Jin
细胞色素 P450 在植物生长中发挥重要作用，并参与多种胁迫反应。然而，人们对细胞色素P450s在苹果中的功能知之甚少。在这里，鉴定了苹果细胞色素 P450 单加氧酶 1 基因 MdCYPM1，并随后从苹果“Gala”（Malus x Domestica）中克隆。为了验证 MdCYPM1 的功能，我们生成了在花椰菜花叶病毒 35S 启动子控制下表达苹果 MdCYPM1 基因的转基因拟南芥植物。选择四个转基因系（#3、#5、#7和#8）用于进一步研究。相对于野生型对照，转基因植物表现出一系列暗形态发生表型，例如叶绿素、花青素含量和下胚轴伸长的减少。此外，MdCYPM1 的过度表达影响转基因拟南芥中生长素的运输和开花时间。此外，盐和甘露醇处理诱导MdCYPM1表达，并且转基因植物在发芽过程中受到盐度和渗透胁迫的负调节。这些结果表明MdCYPM1在植物生长和发育中起着至关重要的作用。 (C) 2017 Elsevier Inc. 保留所有权利。
Cytochrome P450s play an important role in plant growth and are involved in multiple stresses response. However, little is known about the functions of cytochrome P450s in apple. Here, a Malus x domestica cytochrome P450 monooxygenase 1 gene, MdCYPM1, was identified and subsequently cloned from apple 'Gala' (Malus x domestica). To verify the functions of MdCYPM1, we generated transgenic Arabidopsis plants expressing the apple MdCYPM1 gene under the control of the Cauliflower mosaic virus 35S promoter. Four transgenic lines (#3, #5, #7 and #8) were selected for further study. The transgenic plants exhibited a series of skotomorphogenesis phenotypes relative to wild-type controls, such as reduction of the chlorophyll, anthocyanins content and hypocotyls elongation. In addition, overexpression of MdCYPM1 influenced auxin transport and flowering time in transgenic Arabidopsis. Furthermore, MdCYPM1 expression was induced by salt and mannitol treatments, and the transgenic plants were negatively regulated by salinity and osmotic stresses during germination. These results suggest that MdCYPM1 plays a vital role in plant growth and development. (C) 2017 Elsevier Inc. All rights reserved.