Disruption of UDP-galactopyranose mutase expression: A novel strategy for regulation of galactomannan biosynthesis and monascus pigments secretion in Monascus purpureus M9
Disruption of UDP-galactopyranose mutase expression: A novel strategy for regulation of galactomannan biosynthesis and monascus pigments secretion in Monascus purpureus M9
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DOI：
10.1016/j.ijbiomac.2024.129369
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发表时间：
2024-01-16
影响因子：
8.2
通讯作者：
Guo，Qingbin
中科院分区：
文献类型：
--
作者：
Wang，Xufeng;Li，Li;Guo，Qingbin
研究了红曲霉（monascus purpureusM9）细胞壁结构对细胞外红曲色素（exMPs）分泌的影响。为了修饰细胞壁结构，使用农杆菌介导的转化方法敲除了udp -半乳糖酰脲酶（GlfA），导致细胞壁内半乳糖基多糖显著减少。突变后M9的菌丝形态、孢子发生及相关基因表达也发生了变化。在MPs分泌方面，6种exMPs （R1、R2、Y1、Y2、O1和O2）的分泌明显增加。其中，与野生型相比，这些exmp分别提高了1.33倍、1.59倍、0.8倍、2.45倍、2.89倍和4.03倍。这些结果表明，细胞壁结构的改变可能选择性地影响M9中MPs的分泌。并讨论了其基本机制。本研究对孟山都MPs的合成和分泌调控有了新的认识。
The impact of the cell wall structure ofMonascus purpureusM9 on the secretion of extracellular monascus pigments (exMPs) was investigated. To modify the cell wall structure, UDP-galactopyranose mutase (GlfA) was knocked out usingAgrobacterium-mediated transformation method, leading to a significant reduction in the Galf-based polysaccharide within the cell wall. Changes in mycelium morphology, sporogenesis, and the expression of relevant genes in M9 were also observed following the mutation. Regarding MPs secretion, a notable increase was observed in six types of exMPs (R1, R2, Y1, Y2, O1 and O2). Specifically, these exMPs exhibited enhancement of 1.33, 1.59, 0.8, 2.45, 2.89 and 4.03 times, respectively, compared to the wild-type strain. These findings suggest that the alteration of the cell wall structure could selectively influence the secretion of MPs in M9. The underlying mechanisms were also discussed. This research contributes new insights into the regulation of the synthesis and secretion of MPs inMonascusspp..