Photodynamic inactivation of Escherichia coli immobilized on agar surfaces by a tricationic porphyrin
Photodynamic inactivation of Escherichia coli immobilized on agar surfaces by a tricationic porphyrin
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DOI：
10.1016/j.bmc.2006.01.058
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发表时间：
2006-06-15
影响因子：
3.5
通讯作者：
Durantini, Edgardo N.
中科院分区：
文献类型：
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作者：
Caminos, Daniel A.;Durantini, Edgardo N.
5,10,15-三[4-(3-N,N,N-三甲基铵丙氧基)苯基]-20-(4-三氟甲基苯基)碘化卟啉(A(3)B(3+))在固定于琼脂表面的典型革兰氏阴性细菌大肠杆菌上进行了体外光动力活性研究。将三阳离子 A(3)B(3+) 卟啉的结果与 5,10,15,20-四（4-N,N,N-三甲基铵苯基）卟啉对甲苯磺酸盐 (TTAP 41) 的结果进行比较，TTAP 41 是一种标准活性敏化剂，用于根除细胞悬浮液中的大肠杆菌。通过吸光度和荧光的衰减来评估溶液中这些卟啉的光漂白。在这两种情况下，A(3)B(3+) 的光稳定性均高于 TTAp(4+)。在固定于琼脂表面的大肠杆菌细胞中分析了这些敏化剂的光动力灭活能力。小菌落用不同量的敏化剂(0-14nmol)处理并用可见光照射3小时。使用的光源是投影仪或正午的太阳。 A(3)B(3+) 卟啉使表面上的大肠杆菌菌落生长延迟。通过光纤仅照射一个孤立的菌落也获得了类似的结果。在这些条件下，A(3)B(3+) 卟啉表现出高活性，可灭活局部细菌细胞。 A(3)B(3+) 较高的光动力活性通过处理前集落的机械铺展得到证实。该过程使琼脂表面上的大肠杆菌细胞完全失活。因此，三阳离子A(3)B(3+)卟啉是一种有趣的敏化剂，在光动力灭活作为局部感染灶生长的细菌方面具有潜在的应用。 (c) 2006 Elsevier Ltd. 保留所有权利。
The photodynamic activity of 5,10,15-tris[4-(3-N,N,N-trimethylammoniumpropoxy)phenyl]-20-(4-trifluoromethylphenyl)porphyrin iodide (A(3)B(3+)) has been studied in vitro on a typical Gram-negative bacterium Escherichia coli immobilized on agar surfaces. The results obtained for the tricationic A(3)B(3+) porphyrin were compared with those of 5,10,15,20-tetra(4-N,N,N-trimethylammoniumphenyl)porphyrin p-tosylate (TTAP 41), which is a standard active sensitizer established to eradicate E coli in cellular suspension. The photobleaching of these porphyrins in solution was evaluated by decay in absorbance and in fluorescence. In both cases, a higher photostability was found for A(3)B(3+) than for TTAp(4+). Photodynamic inactivation capacities of these sensitizers were analyzed in E coli cells immobilized on agar surfaces. Small colonies were treated with different amount of sensitizer (0-14 nmol) and irradiated with visible light for 3 h. The light source used was either a projector or midday sun. The A(3)B(3+) porphyrin produced a growth delay of E coli colonies on a at surfaces. Similar result was obtained irradiating only one isolated colony through an optical fiber. Under these conditions, A(3)B(3+) porphyrin shows a high activity to inactivate localized bacterial cells. The higher photodynamic activity of A(3)B(3+) was confirmed by mechanical spreading of the colonies before treatment. This procedure produces complete inactivation of E coli cells on the agar surface. Therefore, tricationic A(3)B(3+) porphyrin is an interesting sensitizer with potential applications in photodynamic inactivation of bacteria growing as localized foci of infection. (c) 2006 Elsevier Ltd. All rights reserved.