Fatty acid elongase is required for shoot development in rice
Fatty acid elongase is required for shoot development in rice
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DOI：
10.1111/j.1365-313x.2011.04530.x
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发表时间：
2011-05-01
期刊：
影响因子：
7.2
通讯作者：
Kurata, Nori
中科院分区：
文献类型：
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作者：
Ito, Yukihiro;Kimura, Fumiko;Kurata, Nori
生物体在细胞外覆盖着由各种脂肪酸组成的角质层蜡。在高等植物中，细胞外蜡是保护植物免受物理和生物胁迫（如干旱和病原体攻击）不可缺少的屏障。然而，在正常生长条件下，脂肪酸组成对植物发育的影响尚不清楚。本研究表明，ONION1 （ONI1）基因编码脂肪酸延长酶（β -酮酰辅酶a合成酶），参与超长链脂肪酸的合成，是水稻正确的脂肪酸组成和正常茎部发育所必需的。含有极长链脂肪酸的Oni1突变体产生非常小的芽，具有异常的最外层表皮细胞层，并且在发芽后很快停止生长。这些突变体也表现出KNOX家族同源盒基因的异常表达。ONI1在茎尖分生组织的最外层细胞层和发育中的侧枝器官中特异表达。上述结果表明，脂肪酸延长酶是水稻最外层细胞层形成所必需的，也是水稻全茎发育所不可缺少的细胞层。
P>Organisms are covered extracellularly with cuticular waxes that consist of various fatty acids. In higher plants, extracellular waxes act as indispensable barriers to protect the plants from physical and biological stresses such as drought and pathogen attacks. However, the effect of fatty acid composition on plant development under normal growth conditions is not well understood. Here we show that the ONION1 (ONI1) gene, which encodes a fatty acid elongase (beta-ketoacyl CoA synthase) involved in the synthesis of very-long-chain fatty acids, is required for correct fatty acid composition and normal shoot development in rice. oni1 mutants containing a reduced amount of very-long-chain fatty acids produced very small shoots, with an aberrant outermost epidermal cell layer, and ceased to grow soon after germination. These mutants also showed abnormal expression of a KNOX family homeobox gene. ONI1 was specifically expressed in the outermost cell layer of the shoot apical meristem and developing lateral organs. These results show that fatty acid elongase is required for formation of the outermost cell layer, and this layer is indispensable for entire shoot development in rice.