HIGH-PERFORMANCE AFFINITY-CHROMATOGRAPHY OF DNA
HIGH-PERFORMANCE AFFINITY-CHROMATOGRAPHY OF DNA
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DOI：
10.1016/s0021-9673(00)91270-6
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发表时间：
1990-06-01
期刊：
影响因子：
--
通讯作者：
JARRETT, HW
中科院分区：
文献类型：
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作者：
GOSS, TA;BARD, M;JARRETT, HW
胸腺酸十八聚体（dT）18在5'端磷酸基上以一个初级氨基合成，并与300 . ang共价偶联。孔隙大孔二氧化硅。偶联是在预填充柱内进行活化的n -羟基琥珀酰亚基酯二氧化硅。（dT）18-硅胶柱成功地分离了长度相差一个核苷酸的腺嘌呤低聚物混合物。确定了盐浓度、温度和长度对寡核苷酸洗脱的依赖性。方法也发展选择性地洗脱这样的柱使用盐或温度梯度。
The octadecamer of thymidylic acid, (dT)18, was synthesized with a primary amino group on the 5''-terminal phosphate and this was covalently coupled to 300 .ANG. pore macroporous silica. Coupling was performed inside a prepacked column to an activated N-hydroxysuccinimidyl ester silica. The (dT)18-silica column successfully separates mixtures of adenine oligomers differing in length by one nucleotide. The dependence upon salt concentration, temperature and length for elution of oligonucleotides was determined. Methods were also developed to selectively elute such columns using either salt or temperature gradients.