Real-time detection system for quantification of hepatitis C virus genome
Real-time detection system for quantification of hepatitis C virus genome
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DOI：
10.1016/s0016-5085(99)70185-x
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发表时间：
1999-03-01
期刊：
影响因子：
29.4
通讯作者：
Kohara, M
中科院分区：
文献类型：
--
作者：
Takeuchi, T;Katsume, A;Kohara, M
背景与目的：对于丙型肝炎病毒感染的诊断和患者病毒载量的监测，高度灵敏和准确的丙型肝炎病毒定量系统至关重要。方法：使用 ABI Prism 7700 序列检测器（Perkin Elmer Corp./Applied Biosystems，Foster City，CA）通过实时检测系统检测丙型肝炎病毒基因组。结果：仅检测到 10 个基因组拷贝，定量范围在 10(1) 和 10(8) 个拷贝之间 (r > 0.99)，该系统比 Amplicor 监测仪（新泽西州布兰奇堡的罗氏诊断系统公司）灵敏 10-100 倍。基因组定量鉴定的测定内精度和测定间再现性的变异系数值分别为0.37%至2.00%和0.88%至4.66%。该系统可以检测到98%的慢性肝炎患者、95.8%的肝硬化患者、100%的肝细胞癌患者的基因组，这些患者具有丙型肝炎病毒抗体，但无法检测到没有抗体的患者的基因组。结论：建立实时检测系统可以通过病毒基因组定量更准确地诊断接受干扰素治疗的患者的感染并监测病毒载量。
Background & Aims: For diagnosis of hepatitis C virus infection and monitoring of viral load in patients, a highly sensitive and accurate hepatitis C virus quantification system is essential. Methods: Hepatitis C virus genome was detected by real/time detection system using an ABI Prism 7700 sequence detector (Perkin Elmer Corp./Applied Biosystems, Foster City, CA). Results: AS few as 10 copies of the genome were detected, and the quantification range was between 10(1) and 10(8) copies (r > 0.99), This system tvas 10-100-fold more sensitive than an Amplicor monitor (Roche Diagnostic Systems, Branchburg, NJ). The coefficient of variation values for both intra-assay precision and interassay reproducibility of identifying: the genome quantification ranged from 0.37% to 2.00% and 0.88% to 4.66%, respectively. The system could detect the genome in 98% of patients with chronic hepatitis, 95.8% of patients with liver cirrhosis, and 100% of patients with hepatocellular carcinoma who had the antibody to hepatitis C virus, but could not detect the genome in patients without the antibody. Conclusions: The establishment of a real-time detection system enables more accurate diagnosis of infection and monitoring of viral load in interferon-treated patients via quantification of viral genome.