Tyrosine confounds oxidative electrochemical detection of nitric oxide.
Tyrosine confounds oxidative electrochemical detection of nitric oxide.
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酪氨酸混淆一氧化氮的氧化电化学检测。
DOI：
10.1152/ajpheart.1998.274.5.h1698
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Hanley,DF
中科院分区：
文献类型：
--
作者：
Stingele,R;Wilson,DA;Traystman,RJ;Hanley,DF
我们报告的证据表明，卟啉微传感器检测一氧化氮（NO）也检测生物相关浓度的酪氨酸（Tyr）在狗的大脑。Tyr在与NO相同的电位下被该传感器氧化，并且对NO和Tyr的灵敏度是相同的数量级。来自Tyr的干扰是重要的，因为1）Tyr是丰富的，并且2）血脑屏障中存在Tyr的浓度梯度，如果受到缺血或缺氧的干扰，则可能导致不可预测的结果。这种干扰的知识是很重要的解释与此传感器获得的结果和未来研究的设计。
We report evidence that a porphyrinic microsensor for detection of nitric oxide (NO) also detects biologically relevant concentrations of tyrosine (Tyr) in dog brain. Tyr is oxidized by this sensor at the same potential as NO, and the sensitivity for NO and Tyr are of the same order of magnitude. The interference from Tyr is of importance because1) Tyr is abundant and2) there is a concentration gradient of Tyr across the blood-brain barrier that can lead to unpredictable results if disturbed by ischemia or hypoxia. The knowledge of this interference is important for the interpretation of results obtained with this sensor and for the design of future studies.