Bone Regeneration of Osteoporotic Vertebral Body Defects Using Platelet-Rich Plasma and Gelatin β-Tricalcium Phosphate Sponges.
Bone Regeneration of Osteoporotic Vertebral Body Defects Using Platelet-Rich Plasma and Gelatin β-Tricalcium Phosphate Sponges.
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DOI：
10.1089/ten.tea.2017.0358
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发表时间：
2017-12
影响因子：
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通讯作者：
Munehiro Sakata;H. Tonomura;Tomonori Itsuji;H. Ishibashi;R. Takatori;Y. Mikami;M. Nagae;K. Matsuda;Yasuhiko Tabata;Masaki Tanaka;Toshikazu Kubo
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文献类型：
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作者：
Munehiro Sakata;H. Tonomura;Tomonori Itsuji;H. Ishibashi;R. Takatori;Y. Mikami;M. Nagae;K. Matsuda;Yasuhiko Tabata;Masaki Tanaka;Toshikazu Kubo
本研究的目的是探讨富血小板血浆（PRP）联合明胶β-磷酸三钙（β-TCP）海绵对去卵巢大鼠腰椎椎体缺损骨生成的影响。在前椎体中心产生临界尺寸缺损后，用以下材料填充缺损：（1）无材料（对照组），（2）明胶β-TCP海绵加PRP（PRP海绵组），（3）明胶β-TCP海绵加磷酸盐缓冲盐水（PBS海绵组）。术后立即以及第 4、8 和 12 周进行显微计算机断层扫描和组织学评估，以评估骨再生。术后第12周还进行了生物力学测试。在PRP海绵组中，影像学和组织学检查均显示，与PBS海绵组相比，移植海绵首先诱导可见的成骨，并且观察到骨组织的额外生长。 PRP 海绵或 PBS 海绵移植对缺损周围骨组织的生成没有负面影响。生物力学测试显示 PRP 海绵组受影响椎体的硬度增加。这些结果表明，PRP 浸渍的明胶 β-TCP 海绵可有效促进骨质疏松条件下腰椎骨缺损的骨再生。 PRP 与明胶 β-TCP 海绵相结合可能有助于开发一种治疗骨质疏松性椎体骨折的新椎体成形术方法。
The objective of the present study was to investigate the effect of platelet-rich plasma (PRP) combined with gelatin β-tricalcium phosphate (β-TCP) sponge on bone generation in a lumbar vertebral body defect of ovariectomized rat. After creating critical-size defects in the center of the anterior vertebral body, the defects were filled with the following materials: (1) no material (control group), (2) gelatin β-TCP sponge with PRP (PRP sponge group), and (3) gelatin β-TCP sponge with phosphate-buffered saline (PBS sponge group). Microcomputed tomography and histological evaluation were performed immediately after surgery and at 4, 8, and 12 weeks to assess bone regeneration. Biomechanical test was also performed at postoperative week 12. In the PRP sponge group, both imaging and histological examination showed that visible osteogenesis was first induced and additional growth of bone tissue was observed in the transplanted sponge, compared with the PBS sponge group. There was no negative effect of either PRP sponge or PBS sponge transplantation on bone tissue generation around the periphery of the defect. Biomechanical test showed increased stiffness of the affected vertebral bodies in the PRP sponge group. These results indicate that PRP-impregnated gelatin β-TCP sponge is effective for facilitating bone regeneration in lumbar vertebral bone defect under osteoporotic condition. PRP combined with gelatin β-TCP sponges could be potentially useful for developing a new approach to vertebroplasty for osteoporotic vertebral fracture.