Area postrema neurons are modulated by the adipocyte hormone adiponectin
Area postrema neurons are modulated by the adipocyte hormone adiponectin
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DOI：
10.1523/jneurosci.2014-06.2006
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发表时间：
2006-09-20
影响因子：
5.3
通讯作者：
Ferguson, Alastair V.
中科院分区：
文献类型：
--
作者：
Fry, Mark;Smith, Pauline M.;Ferguson, Alastair V.
脂联素是一种脂肪细胞衍生的肽激素，参与能量稳态和肥胖的发病机制，包括高血压。后脑区（AP）缺乏血脑屏障，是体液和神经信号的重要稳态整合中心。在这里，我们研究AP在脂联素信号传导中的作用。我们发现大鼠AP表达AdipoR1和AdipoR2脂联素受体mRNA。我们使用电流钳电生理学研究脂联素是否影响AP神经元的膜特性，发现约60%的大鼠AP神经元对脂联素敏感。另外的电生理实验结合单细胞逆转录- pcr表明，所有表达两种受体亚型的神经元都对脂联素敏感，而仅表达一种亚型的神经元则主要不敏感。最后，在AP中微量注射脂联素导致动脉血压显著升高，而心率没有变化，这表明脂联素在AP中起作用，在能量稳态控制和心血管功能之间提供了可能的联系。
Adiponectin is an adipocyte-derived peptide hormone involved in energy homeostasis and the pathogenesis of obesity, including hypertension. Area postrema (AP) lacks a blood-brain barrier and is a critical homeostatic integration center for humoral and neural signals. Here we investigate the role of AP in adiponectin signaling. We show that rat AP expresses AdipoR1 and AdipoR2 adiponectin receptor mRNA. We used current-clamp electrophysiology to investigate whether adiponectin influenced membrane properties of AP neurons and found that similar to 60% of rat AP neurons tested were sensitive to adiponectin. Additional electrophysiology experiments coupled with single-cell reverse transcription-PCR indicated that all neurons that expressed both subtypes of receptor were sensitive to adiponectin, whereas neurons expressing only one subtype were predominantly insensitive. Last, microinjection of adiponectin into AP caused significant increases in arterial blood pressure, with no change in heart rate, suggesting that adiponectin acts at AP to provide a possible link between control of energy homeostasis and cardiovascular function.