Alteration of the fecal microbiota and serum metabolite profiles in dogs with idiopathic inflammatory bowel disease.
Alteration of the fecal microbiota and serum metabolite profiles in dogs with idiopathic inflammatory bowel disease.
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DOI：
10.1080/19490976.2014.997612
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发表时间：
2015
期刊：
影响因子：
12.2
通讯作者：
Suchodolski JS
中科院分区：
文献类型：
--
作者：
Minamoto Y;Otoni CC;Steelman SM;Büyükleblebici O;Steiner JM;Jergens AE;Suchodolski JS
特发性炎症性肠病（IBD）是犬慢性胃肠道（GI）疾病的常见原因。潜在的宿主遗传易感性、肠道生态失调和饮食/环境因素的组合被怀疑是犬IBD发病机制的主要促成因素。然而，宿主-微生物相互作用的实际机制仍然难以捉摸。本研究的目的是比较健康犬（n = 10）和IBD犬（n = 12）在药物治疗3周前后的粪便微生物群和血清代谢产物谱。粪便微生物群和代谢产物谱分别通过16 S rRNA基因的454焦磷酸测序和非靶向代谢组学方法进行表征。与健康对照犬相比，在IBD犬中观察到显著较低的细菌多样性和独特的微生物群落。在IBD犬中，虽然γ-变形菌属的比例过高，但丹毒毛菌属、梭菌属和类杆菌属的比例偏低。使用PICRUSt根据16 S rRNA基因数据预测功能基因含量，揭示了IBD犬中细菌分泌系统和转录因子的代表性过高，而氨基酸代谢的代表性不足。IBD犬的血清代谢物3-羟基丁酸、己糖醛酸、核糖和葡萄糖酸内酯显著更丰富。尽管在所有IBD犬中药物治疗后观察到临床改善，但这并不伴随粪便微生物群或血清代谢产物谱的显著变化。这些结果表明，IBD犬存在氧化应激和GI微生物群的功能改变，即使面对对药物治疗的临床反应也会持续存在。
Idiopathic inflammatory bowel disease (IBD) is a common cause of chronic gastrointestinal (GI) disease in dogs. The combination of an underlying host genetic susceptibility, an intestinal dysbiosis, and dietary/environmental factors are suspected as main contributing factors in the pathogenesis of canine IBD. However, actual mechanisms of the host-microbe interactions remain elusive. The aim of this study was to compare the fecal microbiota and serum metabolite profiles between healthy dogs (n = 10) and dogs with IBD before and after 3 weeks of medical therapy (n = 12). Fecal microbiota and metabolite profiles were characterized by 454-pyrosequencing of 16 S rRNA genes and by an untargeted metabolomics approach, respectively. Significantly lower bacterial diversity and distinct microbial communities were observed in dogs with IBD compared to the healthy control dogs. While Gammaproteobacteria were overrepresented, Erysipelotrichia, Clostridia, and Bacteroidia were underrepresented in dogs with IBD. The functional gene content was predicted from the 16 S rRNA gene data using PICRUSt, and revealed overrepresented bacterial secretion system and transcription factors, and underrepresented amino acid metabolism in dogs with IBD. The serum metabolites 3-hydroxybutyrate, hexuronic acid, ribose, and gluconic acid lactone were significantly more abundant in dogs with IBD. Although a clinical improvement was observed after medical therapy in all dogs with IBD, this was not accompanied by significant changes in the fecal microbiota or in serum metabolite profiles. These results suggest the presence of oxidative stress and a functional alteration of the GI microbiota in dogs with IBD, which persisted even in the face of a clinical response to medical therapy.