Infection of tobacco protoplasts with in vitro transcribed tobacco mosaic virus RNA using an improved electroporation method
Infection of tobacco protoplasts with in vitro transcribed tobacco mosaic virus RNA using an improved electroporation method
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DOI：
10.1016/0014-5793(87)81191-2
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发表时间：
1987-07
期刊：
影响因子：
3.5
通讯作者：
Yuichiro Watanabe;T. Meshi;Y. Okada
中科院分区：
文献类型：
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作者：
Yuichiro Watanabe;T. Meshi;Y. Okada
我们以前建立了一个体外转录系统，可以从cDNA模板中产生传染性烟草花叶病毒（TMV） rna [1986] Proc. Natl。学会科学。[j]。在这里，我们报告了一个有用的系统，用于烟草原生质体感染少量的感染性rna，如cDNA模板的体外转录物，涉及改进的电穿孔方法。该系统将有助于利用体外操纵的tmv -RNA阐明基因组RNA及其编码蛋白的功能。
We previously established an in vitro transcription system which can produce infectious tobacco mosaic virus (TMV) RNAs from cDNA templates [(1986) Proc. Natl. Acad. Sci. USA 83, 5043-5047]. Here we report a useful system for the infection of tobacco protoplasts with a small amount of infectious RNAs, like in vitro transcripts from cDNA templates, involving an improved electroporation method. This system will facilitate the elucidation of the functions of the genomic RNA and its coding proteins using in vitro manipulated TMV-RNAs.