Interaction of fibrinogen with its platelet receptor: kinetics and effect of pH and temperature.
Interaction of fibrinogen with its platelet receptor: kinetics and effect of pH and temperature.
复制标题
纤维蛋白原与其血小板受体的相互作用：动力学以及 pH 值和温度的影响。
DOI：
10.1021/bi00508a005
复制
发表时间：
1981
期刊：
影响因子：
2.9
通讯作者：
Plow,EF
中科院分区：
文献类型：
--
作者：
Marguerie,GA;Plow,EF
Gerard A. Marguerie** 和Edward F. Plow* 摘要：纤维蛋白原与人血小板上ADP诱导的受体的相互作用涉及纤维蛋白原的初始可逆结合，随后是血小板结合的纤维蛋白原的稳定。在这项研究中，动力学分析提供了一个独立的表征纤维蛋白原与其血小板受体的可逆结合。此外，还考察了温度和pH值对血小板结合纤维蛋白原的初始可逆相互作用和随后的稳定性的影响。反应的动力学分析表明，血小板刺激，受体诱导，稳定的维生素原受体复合物的associatedevents不是速率决定步骤和纤维蛋白原与血小板的相互作用是充分描述的速率常数的初始可逆结合的纤维蛋白原。在1 mM Ca 2+存在下的结合速率常数k+ h是1 mM Mg 2+存在下的2倍，
Gerard A. Marguerie** and Edward F. Plow* abstract: The interaction of fibrinogen with its ADP-induced receptor on human platelets involves an initial reversible binding of fibrinogen followed by a stabilization of plateletbound fibrinogen. In thisstudy, kinetic analyses were per-formed to provide an independent characterization of the re-versible binding of fibrinogen to its platelet receptor. In ad-dition, the effects of temperature and pH on the initial re-versible interaction and the subsequent stabilization of platelet-bound fibrinogen were examined. Kinetic analysis of the reaction indicated that the associatedevents of platelet stimulation, receptor induction, and stabilization of the fibrino-gen-receptor complex were not rate-determining steps and that the interaction of fibrinogen with platelets was adequately described by the rate constants of the initial reversible binding of fibrinogen. The rate constant of association, k+ h was 2-fold greater in thepresence of 1 mM Ca2+ than of 1 mM Mg2+,