Cell-machine interfaces for characterizing gene regulatory network dynamics.
Cell-machine interfaces for characterizing gene regulatory network dynamics.
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DOI：
10.1016/j.coisb.2019.01.001
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发表时间：
2019-04-01
影响因子：
3.7
通讯作者：
Dunlop, Mary J
中科院分区：
文献类型：
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作者：
Lugagne, Jean-Baptiste;Dunlop, Mary J
基因调控网络及其产生的动态反应提供了有关生物系统如何处理有关其环境的信息的丰富信息。最近，对剖析这些网络感兴趣的研究人员已将其实验工作流程的各个部分外包给计算机。在这里，我们回顾一下如何使用微流体或光遗传学工具与荧光成像相结合，现在可以连接细胞和计算机。这些平台使科学家能够对遗传途径进行信息丰富的动态刺激并监测其反应。还可以实时闭合循环并调节基因，从而提供信号如何通过网络传播的前所未有的视图。最后，我们概述了可以在细胞机器接口框架内使用的新工具。
Gene regulatory networks and the dynamic responses they produce offer a wealth of information about how biological systems process information about their environment. Recently, researchers interested in dissecting these networks have been outsourcing various parts of their experimental workflow to computers. Here we review how, using microfluidic or optogenetic tools coupled with fluorescence imaging, it is now possible to interface cells and computers. These platforms enable scientists to perform informative dynamic stimulations of genetic pathways and monitor their reaction. It is also possible to close the loop and regulate genes in real time, providing an unprecedented view of how signals propagate through the network. Finally, we outline new tools that can be used within the framework of cell-machine interfaces.