DWARF overexpression induces alteration in phytohormone homeostasis, development, architecture and carotenoid accumulation in tomato
DWARF overexpression induces alteration in phytohormone homeostasis, development, architecture and carotenoid accumulation in tomato
复制标题
DWARF 过度表达诱导番茄植物激素稳态、发育、结构和类胡萝卜素积累的改变
DOI：
10.1111/pbi.12474
复制
发表时间：
2016-03-01
影响因子：
13.8
通讯作者：
Yu, Jing-Quan
中科院分区：
文献类型：
--
作者：
Li, Xiao-Jing;Chen, Xiao-Juan;Yu, Jing-Quan
Please try later.
Brassinosteroids (BRs) play a critical role in plant growth, development and stress response; however, genetic evidence for the BR-mediated integrated regulation of plant growth still remains elusive in crop species. Here, we clarified the function of DWARF (DWF), the key BR biosynthetic gene in tomato, in the regulation of plant growth and architecture, phytohormone homeostasis and fruit development by comparing wild type, d(boolean AND im), a weak allele mutant impaired in DWF, and DWF-overexpressing plants in tomato. Results showed that increases in DWF transcripts and endogenous BR level resulted in improved germination, lateral root development, CO2 assimilation and eventually plant growth as characterized by slender and compact plant architecture. However, an increase in DWF transcript down-regulated the accumulation of gibberellin, which was associated with decreases in leaf size and thickness. BRs positively regulated lateral bud outgrowth, which was associated with decreased transcript of Aux/IAA3, and the ethylene-dependent petiole bending and fruit ripening. Notably, overexpression of DWF did not significantly alter fruit yield per plant; however, increases by 57.4% and 95.3% might be estimated in fruit yield per square metre in two transgenic lines due to their compact architecture. Significantly, BR level was positively related with the carotenoid accumulation in the fruits. Taken together, our results demonstrate that BRs are actively involved in the regulation of multiple developmental processes relating to agronomical important traits.