Human infection with Ascaris lumbricoides is associated with suppression of the interleukin-2 response to recombinant cholera toxin B subunit following vaccination with the live oral cholera vaccine CVD 103-HgR
Human infection with Ascaris lumbricoides is associated with suppression of the interleukin-2 response to recombinant cholera toxin B subunit following vaccination with the live oral cholera vaccine CVD 103-HgR
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DOI：
10.1128/iai.69.3.1574-1580.2001
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发表时间：
2001-03-01
影响因子：
3.1
通讯作者：
Nutman, TB
中科院分区：
文献类型：
--
作者：
Cooper, PJ;Chico, M;Nutman, TB
为了研究同时使用蛔虫素对活口服疫苗的细胞因子应答的潜在免疫调节作用，我们测量了随机分为两组的蛔虫感染受试者接种口服霍乱活疫苗CVD 103-HgR后对霍乱毒素B亚基（CT-B）的细胞因子反应在接种疫苗前接受两剂阿苯达唑或安慰剂的盲法研究和一组健康的美国对照。接种后对CT-B的细胞因子反应的特征是三个研究组中白细胞介素-2（IL-2; P = 0.02）和γ干扰素（IFN-γ; P = 0.001）产生的短暂增加;然而，仅在阿苯达唑治疗的A，蛔虫感染组中IFN-γ的接种后增加显著（P = 0.008）。与安慰剂组相比，阿苯达唑治疗组的接种后IL-2水平显著更高对照组Th 1和Th 2细胞因子水平无明显变化（P = 0.03），提示CVD 103-HgR疫苗接种与Th 1细胞因子应答有关（IL-2和IFN-γ）对CT-B的应答，感染蛔虫可降低这种应答的程度，接种疫苗前的阿苯达唑治疗能够部分逆转IL-2的缺乏，地蠕虫寄生虫对口服疫苗免疫应答的潜在调节对疫苗接种的设计具有重要意义在土蠕虫流行地区开展运动。
To investigate the potential immunomodulatory effects of concurrent ascariasis on the cytokine response to a live oral vaccine, we measured cytokine responses to cholera toxin B subunit (CT-B) following vaccination with the live oral cholera vaccine CVD 103-HgR in Ascaris lumbricoides-infected subjects randomized in a double-blind study to receive two doses of either albendazole or placebo prior to vaccination and in a group of healthy U.S. controls. Postvaccination cytokine responses to CT-B were characterized by transient increases in the production of interleukin-2 (IL-2; P = 0.02) and gamma interferon (IFN-gamma; P = 0.001) in the three study groups combined; however, postvaccination increases in IFN-gamma were significant only in the albendazole-treated A, lumbricoides infection group (P = 0.008). Postvaccination levels of IL-2 were significantly greater in the albendazole-treated group compared with the placebo group (P = 0.03), No changes in levels of Th1 and Th2 cytokines in response to control ascaris antigens were observed over the same period, These findings indicate that vaccination with CVD 103-HgR is associated with a Th1 cytokine response (IL-2 and IFN-gamma) to CT-B, that infection with A, lumbricoides diminishes the magnitude of this response, and that albendazole treatment prior to vaccination was able to partially reverse the deficit in IL-2, The potential modulation of the immune response to oral vaccines by geohelminth parasites has important implications for the design of vaccination campaigns in geohelminth-endemic areas.