CHARACTERIZATION OF NEURONS IN THE CORTICAL WHITE MATTER IN HUMAN TEMPORAL LOBE EPILEPSY
CHARACTERIZATION OF NEURONS IN THE CORTICAL WHITE MATTER IN HUMAN TEMPORAL LOBE EPILEPSY
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DOI：
10.1016/j.neuroscience.2016.07.011
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发表时间：
2016-10-01
期刊：
影响因子：
3.3
通讯作者：
Abraham, Hajnalka
中科院分区：
文献类型：
--
作者：
Richter, Zsofia;Janszky, Jozsef;Abraham, Hajnalka
目前工作的目的是表征古皮质和新皮质白质中的神经元，并研究它们在颞叶内侧硬化中的分布。对治疗抵抗性颞叶癫痫患者手术切除的组织切片进行免疫组织化学和神经元定量。将肿瘤但无癫痫患者的颞叶组织和尸检的颞叶组织用作对照。使用抗 NeuN、钙结合蛋白、转录因子 Tbr1 和神经丝的抗体通过免疫组织化学鉴定神经元。我们发现颞叶癫痫患者的古皮质和新皮质白质中的神经元密度明显高于对照组。根据其形态和神经化学成分，这些神经元中同时存在兴奋性和抑制性细胞。白质中的一部分神经元具有 Tbr-1 免疫反应性，这些神经元共表达 NeuN 和神经丝标记物 SMI311R。没有观察到 Tbr1 与抑制性神经元标记物、钙结合蛋白的共定位。我们认为大量的白质神经元包含了亚板的残余物。此外，我们提出白质神经元的一个子集在迁移过程中被抑制，突出了皮质发育不良在与颞叶内侧硬化相关的癫痫中的作用。 (C) 2016 国际广播组织。由爱思唯尔有限公司出版。保留所有权利。
The aim of the present work was to characterize neurons in the archi- and neocortical white matter, and to investigate their distribution in mesial temporal sclerosis. Immunohistochemistry and quantification of neurons were performed on surgically resected tissue sections of patients with therapy-resistant temporal lobe epilepsy. Temporal lobe tissues of patients with tumor but without epilepsy and that from autopsy were used as controls. Neurons were identified with immunohistochemistry using antibodies against NeuN, calcium-binding proteins, transcription factor Tbr1 and neurofilaments. We found significantly higher density of neurons in the archi- and neocortical white matter of patients with temporal lobe epilepsy than in that of controls. Based on their morphology and neurochemical content, both excitatory and inhibitory cells were present among these neurons. A subset of neurons in the white matter was Tbr-1-immunoreactive and these neurons coexpressed NeuN and neurofilament marker SMI311R. No colocalization of Tbr1 was observed with the inhibitory neuronal markers, calcium-binding proteins. We suggest that a large population of white matter neurons comprises remnants of the subplate. Furthermore, we propose that a subset of white matter neurons was arrested during migration, highlighting the role of cortical maldevelopment in epilepsy associated with mesial temporal sclerosis. (C) 2016 IBRO. Published by Elsevier Ltd. All rights reserved.