Genetic diversity of three classes of integrons in antibiotic-resistant bacteria isolated from Jiulong River in southern China
Genetic diversity of three classes of integrons in antibiotic-resistant bacteria isolated from Jiulong River in southern China
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DOI：
10.1007/s11356-015-4480-0
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发表时间：
2015-04
影响因子：
5.8
通讯作者：
Mao Lin;Jingjing Liang;Xian Zhang;Xiao-Min Wu;Q. Yan;Zhuanxi Luo
中科院分区：
文献类型：
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作者：
Mao Lin;Jingjing Liang;Xian Zhang;Xiao-Min Wu;Q. Yan;Zhuanxi Luo
We identified antibiotic-resistant bacterial isolates from the surface waters of Jiulong River basin in southern China and determined their extent of resistance, as well as the prevalence and characterization of three classes of integrons. A phylogenetic analysis of 16S ribosomal DNA (rDNA) sequences showed that 20 genera were sampled from a total of 191 strains and the most common genus wasAcinetobacter. Antimicrobial susceptibility testing revealed that the 191 isolates were all multiresistant and there were high levels of resistance to 19 antimicrobials that were tested, particularly the β-lactam, sulfonamide, amphenicol, macrolide, and rifamycin classes. Moreover, class 1 integrons were ubiquitous while only five out of 191 strains harbored class 2 integrons and no class 3 integrons were detected. The variable region of the class 1 integrons contained 30 different gene cassette arrays. Nine novel arrays were found in 65 strains, and seven strains had empty integrons. Among these 30 arrays, there were 34 different gene cassettes that included 25 resistance genes, six genes with unknown functions, two mutant transposase genes, and a new gene. The unique arraydfrA1-sat2-aadA1was detected in all five isolates carrying the class 2 integron. We found that antibiotic-resistant bacterial isolates from Jiulong River were diverse and antibiotic resistance genes associated with integrons were widespread.
