Neuroepithelial and ependymal changes in HTX rats with congenital hydrocephalus: An ultrastructural and immunohistochemical study
Neuroepithelial and ependymal changes in HTX rats with congenital hydrocephalus: An ultrastructural and immunohistochemical study
复制标题
先天性脑积水 HTX 大鼠的神经上皮和室管膜变化：超微结构和免疫组织化学研究
DOI：
10.1111/j.1440-1827.1998.tb03880.x
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发表时间：
1998
影响因子：
2.2
通讯作者：
K. Mori
中科院分区：
文献类型：
--
作者：
Yuji Nojima;H. Enzan;Y. Hayashi;H. Nakayama;H. Kiyoku;M. Hiroi;K. Mori
为了探讨先天性脑积水的发病机制，对16天至4周的HTX大鼠和同龄正常Wlstar大鼠的大脑进行了检查。在胎HTX大鼠脑中，侧脑室从妊娠20天开始对称扩张。脑室周围神经上皮变薄，细胞排列紊乱，变形。在HTX大鼠脑的侧脑室中也发现了类似的神经上皮异常，在妊娠20天时没有脑积水的肉眼可见体征。神经上皮细胞扁平，细胞间隙增宽，脱落细胞侧面有微绒毛形成，并有大量巨噬细胞浸润。另一方面，第三脑室和导水管的神经上皮细胞受到的影响不太严重，或没有显示出显着的异常。免疫组化结果显示，出生前后脑积水HTX大鼠侧脑室神经上皮和室管膜大多呈波形蛋白阳性，而正常对照组侧脑室神经上皮和室管膜均呈少量或无波形蛋白阳性。这些形态学变化表明，侧脑室神经上皮的优先参与可能与HTX大鼠先天性脑积水的发病机制密切相关。
To Investigate the pathogenesis of congenital hydrocephalus the brains of HTX rats aged between 16 days and 4 weeks and the brains of normal Wlstar rats of the same ages were examined. In the fetal HTX rat brains, the lateral ventricles were symmetrically dilated from 20 days of gestation. The neuroeplthelium bordering the ventricles showed thinning with cellular disarrangement and deformity. Similar neuroepithelial abnormalities were also found in the lateral ventricles of the HTX rat brain with no macroscopic signs of hydrocephalus at 20 days of gestation. The neuroepithelium showed flattening of the cells, widening of the intercellular spaces, formation of microvilli on the detached lateral cell surfaces, and frequent macrophage infiltration. On the other hand, the neuroepithelial cells of the third ventricle and the aqueduct were affected less severely or showed no significant abnormalities. Immunohistochemlcally, most of the neuroepithelium and ependyma of the lateral ventricles were positive for vimentin in both prenatal and postnatal hydrocephalic HTX rats, while a small number or none of those in normal control rats were positive. These morphological changes suggested that preferential involvement of the lateral ventricular neuroepithelium might be closely associated with the pathogenesis of congenital hydrocephalus in HTX rats.