Accuracy of polymerase chain reaction assays for diagnosis of feline immunodeficiency virus infection in cats
Accuracy of polymerase chain reaction assays for diagnosis of feline immunodeficiency virus infection in cats
复制标题
DOI：
10.2460/javma.2005.226.1503
复制
发表时间：
2005-05-01
影响因子：
1.9
通讯作者：
Levy, JK
中科院分区：
文献类型：
--
作者：
Crawford, PC;Slater, MR;Levy, JK
目的-确定商业诊断实验室提供的用于诊断猫FIV感染的聚合酶链反应（PCB）测定的灵敏度、特异性和总体诊断准确性。设计-前瞻性临床试验。动物-124只猫。程序-从既未感染FIV也未接种FIV的猫、接种许可的FIV疫苗的未感染猫、以及用代表A、B和C亚型的FIV实验性和自然感染的猫。将编码的血液样本提交给美国和加拿大的3个实验室，为兽医提供用于诊断FIV感染的PCR检测。所有实验室都检测了新鲜血液样本，一个实验室还检测了作为干血涂片提交的样本。通过病毒分离确认所有猫的FIV感染状态。灵敏度，特异性和正确的结果进行了计算，为每个PCR assay.Results-Sensitivity范围从41%到93%。未接种猫的特异性范围为81%-100%，接种抗FIV疫苗的猫的特异性范围为44%-95%。在124只测试猫中，58%至90%的猫获得了正确的结果。所有测试都错误地识别了未感染和感染的猫。所有实验室的假阳性结果在接种FIV疫苗的猫中高于未接种疫苗的猫，这表明疫苗接种会干扰用于诊断FIV感染的PCR检测的性能或解释。结论和临床相关性-目前在北美销售给兽医的用于诊断FIV感染的PCR检测在诊断准确性方面差异很大，并且没有解决诊断难题这是因为猫接种了FIV疫苗。
Objective-To determine the sensitivity, specificity, and overall diagnostic accuracy of polymerase chain reaction (PCB) assays offered by commercial diagnostic laboratories for diagnosis of FIV infection in cats.Design-Prospective clinical trial.Animals-124 cats.Procedure-Blood was collected from cats that were neither infected with nor vaccinated against FIV, uninfected cats that were vaccinated with a licensed FIV vaccine, and cats experimentally and naturally infected with FIV representing subtypes A, B, and C. Coded blood samples were submitted to 3 laboratories in the United States and Canada offering PCR assays for diagnosis of FIV infection to veterinary practitioners. All laboratories tested fresh blood samples, and 1 laboratory also tested samples submitted as dried blood smears. The FIV infection status in all cats was confirmed by virus isolation. Sensitivity, specificity, and correct results were calculated for each PCR assay.Results-Sensitivity ranged from 41% to 93%. Specificity ranged from 81% to 100% in unvaccinated cats and 44% to 95% in cats vaccinated against FIV. Correct results were obtained in 58% to 90% of 124 cats tested. All tests misidentified both uninfected and infected cats. False-positive results by all laboratories were higher in cats vaccinated against FIV than in unvaccinated cats, suggesting that vaccination interferes with the performance or interpretation of PCR assays used for diagnosis of FIV infection.Conclusions and Clinical Relevance-PCR assays used for diagnosis of FIV infection presently marketed to veterinary practitioners in North America vary significantly in diagnostic accuracy and did not resolve the diagnostic dilemma resulting from vaccination of cats against FIV.