Enkephalin induces Ca2+ mobilization in single cells of bradykinin-sensitized differentiated neuroblastoma hybridoma (NG108-15) cells
Enkephalin induces Ca2+ mobilization in single cells of bradykinin-sensitized differentiated neuroblastoma hybridoma (NG108-15) cells
复制标题
脑啡肽诱导缓激肽致敏分化神经母细胞瘤杂交瘤 (NG108-15) 细胞单细胞中 Ca2+ 动员
DOI：
10.1016/0304-3940(92)90812-l
复制
发表时间：
1992
影响因子：
2.5
通讯作者：
Y. Kondo
中科院分区：
文献类型：
--
作者：
H. Tomura;F. Okajima;Y. Kondo
应用Fura-2视频显像技术研究了分化的神经母细胞瘤×胶质瘤杂交细胞（NG 108 -15）对脑啡肽（EK）的胞内Ca ~（2+）反应。单用EK对单细胞[Ca ~（2+）] i无影响。然而，阿片样物质诱导[Ca ~（2+）] i rise，当细胞与缓激肽（BK）孵育前EK处理。百日咳毒素处理完全消除了EK对分化的杂交瘤细胞的BK辅助刺激。这些结果表明，在单个NG 108 -15细胞中，EK诱导Ca 2+动员，这是通过EK和BK受体系统之间的串扰通过百日咳毒素敏感的G蛋白的帮助。
A study of the intracellular Ca2+([Ca2+]i) response of differentiated neuroblastoma × glioma hybrid cells (NG108-15 cell) to enkephalin (EK) was carried out by fura-2 video-imaging. EK alone did not influence [Ca2+]iin single cells. The opioid did, however, induce a marked [Ca2+]irise, when the cells were incubated with bradykinin (BK) prior to the EK treatment. Such BK-assisted stimulation of the differentiated hybridoma cells by EK was completely abolished by pertussis toxin treatment. These results suggest that in single NG108-15 cells, EK induces Ca2+mobilization which is assisted by cross-talk between the EK and BK receptor systems via a pertussis toxin-sensitive G protein.
神经母细胞瘤-神经胶质瘤杂交 NG108-15 细胞中脑啡肽和生长抑素阻断钙通道。
DOI：
10.1073/pnas.83.24.9832
发表时间：
1986
影响因子：
11.1
作者：
Tsunoo,A;Yoshii,M;Narahashi,T
通讯作者：
Narahashi,T