A label-free fluorescent molecular switch for Cu2+ based on metal ion-triggered DNA-cleaving DNAzyme and DNA intercalator
A label-free fluorescent molecular switch for Cu2+ based on metal ion-triggered DNA-cleaving DNAzyme and DNA intercalator
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基于金属离子触发DNA切割DNAzyme和DNA嵌入剂的Cu2+无标记荧光分子开关
DOI：
10.1039/c3nj41103f
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发表时间：
2013-03
影响因子：
3.3
通讯作者：
Yao, Shouzhuo
中科院分区：
文献类型：
--
作者：
Wei, Mingjie;Yi, Yinhui;Li, Haitao;Yao, Shouzhuo
基于DNA内切割和DNA嵌入剂SYBR绿色I，构建了一种检测Cu 2+的无标记荧光传感器。由酶链和底物链杂交形成的DNA酶复合物具有两个碱基配对区，包括DNA双链体和DNA三链体。铜离子诱导的催化反应使底物链在切割位点不可逆地断裂，这干扰了DNA链的形成。弱荧光染料SYBR绿色I作为荧光指示剂，插入DNA酶的碱基配对区域，导致强荧光发射。在测试系统中存在Cu 2+的情况下，互补区域由于DNA链断裂而减少。由于SYBR绿色I从切割区域释放，荧光强度降低。其他金属离子在50倍Cu ~（2+）浓度下也没有明显的“开关”现象。在最佳条件下，Cu 2+浓度在4 × 10−8 ~ 120 × 10−8 M范围内呈线性响应。检测限低至10 nM。该传感器已成功应用于饮用水中Cu 2+的测定。
A label-free fluorescent sensor for the detection of Cu2+ was constructed based on internal DNA cleavage and DNA intercalator SYBR Green I. The DNAzyme complex formed by the hybridization of the enzyme strand and substrate strand has two base-pairing regions including a DNA duplex and a DNA triplex. The copper ion-induced catalytic reaction makes the substrate strand irreversibly break at the cleavage site, which disturbs the formation of DNA strands. The weakly fluorescent dye SYBR Green I served as a fluorescence indicator that intercalated into the base-pairing regions of DNAzyme, resulting in a strong fluorescence emission. In the presence of Cu2+ in the test system, the complementary regions were reduced due to the fracture of the DNA strand. The fluorescence intensity was decreased because SYBR Green I was released from the cleaved regions. Other metal ions had no notable “on-off” phenomenon even at 50 times Cu2+ concentration. Under the optimal conditions, a linear response with Cu2+ concentration ranging from 4 × 10−8 to 120 × 10−8 M was obtained. The detection limit reached as low as 10 nM. Moreover, the cost-effective sensor was successfully applied to the determination of Cu2+ in drinking water.
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