Muscle cell attachment in Caenorhabditis elegans.
Muscle cell attachment in Caenorhabditis elegans.
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DOI：
10.1083/jcb.114.3.465
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发表时间：
1991-08
期刊：
影响因子：
--
通讯作者：
Waterston RH
中科院分区：
文献类型：
--
作者：
Francis R;Waterston RH
在秀丽隐杆线虫中，体壁肌肉对角质层施加力以产生运动。在肌细胞和角质层之间是基底膜和薄的皮下细胞。后者包含附着在皮下细胞膜致密斑块上的纤维束，我们称之为纤维细胞器。为了确定将肌肉细胞固定在角质层上的分子链，我们使用富含这些成分的制剂分离出了5个单抗。两种抗体确定200-kD肌肉抗原，可能是肌肉/皮下界面基膜的一部分。另外三种抗体可能识别邻近皮下纤维细胞器的成分。与哺乳动物中间细丝发生反应的mAb IFA也能识别这些结构。我们认为，这些抗体识别的成分可能参与了从肌肉细胞到角质层的张力传递。
In the nematode Caenorhabditis elegans, the body wall muscles exert their force on the cuticle to generate locomotion. Interposed between the muscle cells and the cuticle are a basement membrane and a thin hypodermal cell. The latter contains bundles of filaments attached to dense plaques in the hypodermal cell membranes, which together we have called a fibrous organelle. In an effort to define the chain of molecules that anchor the muscle cells to the cuticle we have isolated five mAbs using preparations enriched in these components. Two antibodies define a 200-kD muscle antigen likely to be part of the basement membrane at the muscle/hypodermal interface. Three other antibodies probably identify elements of the fibrous organelles in the adjacent hypodermis. The mAb IFA, which reacts with mammalian intermediate filaments, also recognizes these structures. We suggest that the components recognized by these antibodies are likely to be involved in the transmission of tension from the muscle cell to the cuticle.