Exploring the vascular smooth muscle receptor landscape in vivo: ultrasound Doppler versus near-infrared spectroscopy assessments.
Exploring the vascular smooth muscle receptor landscape in vivo: ultrasound Doppler versus near-infrared spectroscopy assessments.
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探索体内血管平滑肌受体景观：超声多普勒与近红外光谱评估。
DOI：
10.1152/ajpheart.00782.2013
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Wray,DWalter
中科院分区：
文献类型：
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作者：
Ives,StephenJ;Fadel,PaulJ;Brothers,RMatthew;Sander,Mikael;Wray,DWalter
超声多普勒和近红外光谱（NIRS）常规用于无创监测外周血流动力学在临床和实验设置。然而，这些方法的比较能力，以检测微血管和整个肢体血流动力学的变化，在位于动脉树内的不同位置的血管平滑肌受体的药理学操作是未知的。因此，在10名健康受试者中，（25 ± 2岁），静息腿部血流变化（超声多普勒;股动脉）和肌肉氧合（氧合血红蛋白+氧合肌红蛋白;股外侧肌）对苯肾上腺素动脉内输注的反应（PE，0.025-0.8 μg·kg−1·min−1）、BHT-933（2.5-40 μg·kg−1·min−1）和血管紧张素II（ANG II，0.5-8 ng·kg−1·min−1）。所有药物均引起腿部血流量和氧合血红蛋白+氧合肌红蛋白的显著剂量依赖性降低。在PE（r2= 0.37 ± 0.08）、BHT-933（r2= 0.74 ± 0.06）和ANG II（r2= 0.68 ± 0.13）剂量下，发现超声多普勒和NIRS变化之间存在显著相关性，其中与优先位于腿部微循环“下游”的受体激动剂（BHT-933和ANG II）的相关性最强。药物效力分析显示，PE（0.06 ± 0.02 vs. 0.10 ± 0.01）、BHT-933（5.0 ± 0.9 vs. 4.5 ± 1.3）和ANG II（1.4 ± 0.8 vs. 1.3 ± 0.3）的超声多普勒和NIRS测量值之间的EC 50相似。这些数据提供的证据表明，超声多普勒和近红外光谱跟踪诱导的外周血液动力学变化，并同样能够确定药物效力。然而，在靶向不同水平动脉树的激动剂输注之间观察到相当大的差异，表明受体景观是正确解释这些方法的血流动力学监测的重要考虑因素。
Ultrasound Doppler and near-infrared spectroscopy (NIRS) are routinely used for noninvasive monitoring of peripheral hemodynamics in both clinical and experimental settings. However, the comparative ability of these methodologies to detect changes in microvascular and whole limb hemodynamics during pharmacological manipulation of vascular smooth muscle receptors located at varied locations within the arterial tree is unknown. Thus, in 10 healthy subjects (25 ± 2 yr), changes in resting leg blood flow (ultrasound Doppler; femoral artery) and muscle oxygenation (oxyhemoglobin + oxymyoglobin; vastus lateralis) were simultaneously evaluated in response to intra-arterial infusions of phenylephrine (PE, 0.025–0.8 μg·kg−1·min−1), BHT-933 (2.5–40 μg·kg−1·min−1), and angiotensin II (ANG II, 0.5–8 ng·kg−1·min−1). All drugs elicited significant dose-dependent reductions in leg blood flow and oxyhemoglobin + oxymyoglobin. Significant relationships were found between ultrasound Doppler and NIRS changes across doses of PE (r2= 0.37 ± 0.08), BHT-933 (r2= 0.74 ± 0.06), and ANG II (r2= 0.68 ± 0.13), with the strongest relationships evident with agonists for receptors located preferentially “downstream” in the leg microcirculation (BHT-933 and ANG II). Analyses of drug potency revealed similar EC50between ultrasound Doppler and NIRS measurements for PE (0.06 ± 0.02 vs. 0.10 ± 0.01), BHT-933 (5.0 ± 0.9 vs. 4.5 ± 1.3), and ANG II (1.4 ± 0.8 vs. 1.3 ± 0.3). These data provide evidence that both ultrasound Doppler and NIRS track pharmacologically induced changes in peripheral hemodynamics and are equally capable of determining drug potency. However, considerable disparity was observed between agonist infusions targeting different levels of the arterial tree, suggesting that receptor landscape is an important consideration for proper interpretation of hemodynamic monitoring with these methodologies.