Objective Measurement of Local Rod and Cone Function Using Gaze-Controlled Chromatic Pupil Campimetry in Healthy Subjects
Objective Measurement of Local Rod and Cone Function Using Gaze-Controlled Chromatic Pupil Campimetry in Healthy Subjects
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DOI：
10.1167/tvst.8.6.19
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发表时间：
2019-11-01
影响因子：
3
通讯作者：
Stingl, Krunoslav
中科院分区：
文献类型：
--
作者：
Kelbsch, Carina;Stingl, Katarina;Stingl, Krunoslav
目的：介绍了一种新的视控彩色瞳孔测量（CPC）方法，通过测量瞳孔对局部刺激的反应来绘制视杆和视锥细胞活动的功能图。方法：对14例健康受试者（男性4例，34±11岁，平均6标准差[SD]）在30度中央视野范围内不同位置局部光和暗光刺激下瞳孔收缩幅度和收缩发作潜伏期进行分析。所有受试者均测量两次，以评估该方法的重测变异性和可重复性。结果：ConeProt方案中，相对最大收缩幅度在中心最为明显（26.8% +/- 6.3%），从中央凹到周围呈小山状缩小。对于偏向杆的协议（RodProt），它更小（中心，13.5% +/- 4.5%），轮廓缺少中心峰。锥细胞到收缩发作的平均潜伏期（277 +/- 25 ms）比杆状细胞快（372 +/- 13 ms）。RodProt在不同刺激位置的平均类内相关性为0.84 +/- 0.08，ConeProt为0.75 +/- 0.11；各刺激部位重复性值的平均系数分别为5.9% +/- 1.2%和8.6% +/- 1.7%。结论：CPC能客观评价中央30度视野范围内的局部视杆和视锥功能。它显示了健康受试者的光感受器特异性特征。由于其简便、非接触、眼球控制的特点，是一种临床适用的方法，可以填补人眼视网膜视杆细胞和视锥细胞功能诊断的空白。
Purpose: We introduce a new approach for functional mapping of rod and cone activity by measuring pupillary responses to local stimulation via gaze-controlled chromatic pupil campimetry (CPC).Methods: Pupillary constriction amplitude and latency to constriction onset to local photopic and scotopic light stimuli at different locations within the 30 degrees central visual field were analyzed in 14 healthy subjects (4 males, 34 +/- 11 years, mean 6 standard deviation [SD]). All subjects were measured twice for evaluating the test-retest variability and reproducibility of the method.Results: For the cone-favoring protocol (ConeProt), the relative maximal constriction amplitude was most pronounced in the center (26.8% +/- 6.3%) with a hill-shaped decrease from the fovea to the periphery. For the rod-favoring protocol (RodProt), it was smaller (center, 13.5% +/- 4.5%) with a profile lacking the central peak. Mean latency to constriction onset was faster for cones (277 +/- 25 ms) than for rods (372 +/- 13 ms). Mean intraclass correlation at the different stimulus locations was 0.84 +/- 0.08 for RodProt and 0.75 +/- 0.11 for ConeProt; mean coefficients of repeatability value of all stimulus locations was 5.9% +/- 1.2% and 8.6% +/- 1.7%, respectively.Conclusions: CPC provides an objective evaluation of local rod and cone function within the central 30 degrees visual field. It shows a photoreceptor-specific profile in healthy subjects. Due to its easy, noncontact, gaze-controlled character, it is a clinically applicable method and may fill the gap of functional diagnostics of rods and cones of the human retina.