Organization of cortico-cortical associative projections in rats exposed to ethanol during early postnatal life
Organization of cortico-cortical associative projections in rats exposed to ethanol during early postnatal life
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DOI：
10.1016/s0361-9230(03)00052-2
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发表时间：
2003-05-30
影响因子：
3.8
通讯作者：
Giannetti, S
中科院分区：
文献类型：
--
作者：
Granato, A;Di Rocco, F;Giannetti, S
在出生后第一周暴露于乙醇吸入的成年大鼠中，研究了皮质-皮质关联投射的精细组织。乙醇处理和对照动物接受皮质注射生物素化葡聚糖胺结合N-甲基-D-天冬氨酸，以获得高尔基体样逆行标记的联合锥体神经元。从标记分析中获得的结果可以概括如下：（a）乙醇处理的动物中的联合投射神经元比正常动物中的少;（B）乙醇处理的动物中的颗粒上和颗粒下联合神经元的数目之间的比率比对照组高;（c）与对照组相比，在乙醇暴露的情况下，第2/3层锥体联合细胞的基底树突显示简化的树突分支;（d）聚类分析显示，正常树突可根据其几何特性明确地细分为不同的组，而暴露于乙醇的动物的树突遵循不太稳健的分组标准。这些差异进行了讨论，考虑功能的改变，胎儿酒精综合征的特点。(C)2003年爱思唯尔科学公司All rights reserved.
The fine organization of cortico-cortical associative projections was investigated in adult rats exposed to inhalation of ethanol during the first postnatal week. Ethanol-treated and control animals received cortical injections of biotinylated dextran amine combined with N-methyl-D-aspartic acid, in order to obtain a Golgi-like retrograde labeling of associative pyramidal neurons. The results obtained from the analysis of labeling can be summarized as follows: (a) there are fewer associative projection neurons in ethanol-treated than in normal animals; (b) the ratio between the number of supragranular and infragranular associative neurons is higher in ethanol-treated animals compared to controls; (c) the basal dendrites of pyramidal associative cells of layer 2/3 display a simplified dendritic branching in ethanol exposed cases as compared to controls; (d) the cluster analysis shows that normal dendrites can be clearly subdivided into different groups according to their geometric properties, whereas dendrites from animals exposed to ethanol follow less robust grouping criteria. These differences are discussed in consideration of the functional alterations that characterize the fetal alcohol syndrome. (C) 2003 Elsevier Science Inc. All rights reserved.