NUCLEAR TRANSPORT OF INFLUENZA-VIRUS POLYMERASE PA PROTEIN
NUCLEAR TRANSPORT OF INFLUENZA-VIRUS POLYMERASE PA PROTEIN
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DOI：
10.1016/0168-1702(92)90031-4
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发表时间：
1992-06-01
期刊：
影响因子：
5
通讯作者：
ORTIN, J
中科院分区：
文献类型：
--
作者：
NIETO, A;DELALUNA, S;ORTIN, J
已使用SV 40重组病毒（SVPA 76）研究了流感聚合酶PA亚单位的亚细胞分布，该重组病毒允许该蛋白在COS-1细胞中表达和积累。与流感病毒感染后几小时观察到的PA亚基的完全核定位相反，当COS-1细胞被SVPA 76重组体感染时，PA蛋白在细胞核、细胞质中积累或分布在整个细胞中。当用SVPA 76重组体感染细胞并用流感病毒超感染时，观察到显示PA蛋白质核定位的细胞比例明显增加，表明可能需要一些反式因子以允许蛋白质的完全核积累。用SVPA 76重组体和SVPB 1或SVNS重组体病毒的双重感染显示聚合酶PB 1亚基或NS 1蛋白的表达与聚合酶PA亚基的核定位之间完全相关。然而，在SVPA 76和SVNP重组体的双重感染中没有观察到这种相关性。这些结果表明，聚合酶PB 1亚基和非结构蛋白可能参与流感病毒聚合酶PA蛋白的核靶向或核滞留。
The subcellular distribution of influenza polymerase PA subunit has been studied using a SV40-recombinant virus (SVPA76), which allows the expression and accumulation of this protein in COS-1 cells. In contrast to the complete nuclear localization observed for the PA subunit several hours after influenza virus infection, when COS-1 cells were infected with the SVPA76 recombinant, the PA protein accumulated either in the nucleus, in the cytoplasm or was distributed throughout the cell. When cells were infected with the SVPA76 recombinant and superinfected with influenza virus, a clear increase in the proportion of cells showing nuclear localization of the PA protein was observed, suggesting that some trans-factor may be required to allow complete nuclear accumulation of the protein. Double infections using SVPA76 recombinant and either SVPB1 or SVNS recombinant viruses showed a complete correlation between expression of polymerase PB1 subunit or NS1 protein and nuclear localization of polymerase PA subunit. However, no such correlation was observed in the double infections of SVPA76 and SVNP recombinants. These results suggest that polymerase PB1 subunit and the non-structural proteins could be involved in the nuclear targeting or nuclear retention of influenza polymerase PA protein.