Differential expression of retinoic acid-synthesizing (RALDH) enzymes during fetal development and organ differentiation in the mouse
Differential expression of retinoic acid-synthesizing (RALDH) enzymes during fetal development and organ differentiation in the mouse
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DOI：
10.1016/s0925-4773(01)00561-5
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发表时间：
2002-01-01
影响因子：
2.6
通讯作者：
Dollé, P
中科院分区：
文献类型：
--
作者：
Niederreither, K;Fraulob, V;Dollé, P
三种催化视黄醇氧化成视黄酸的视黄醇脱氢酶（RALDH 1、RALDH 2和RALDH 3）已显示在早期胚胎发生期间差异表达。在这里，我们报告他们的差异表达模式在整个后期小鼠器官发生。Raldh1在发育中的肺中显著表达（特别是在支气管和气管上皮中），并且在胃和肠上皮和间充质层中显示阶段特异性表达。Raldh3表达对分化中的肠固有层具有特异性。Raldh2在整个肾生肾区表达，而Raldh1和Raldh3主要在集合管上皮中表达。Raldh3的表达比Raldh1在泌尿生殖道和性腺上皮中的表达更受限制，而Raldh2的表达是间充质的。Raldh1与Raldh2在早期心脏心外膜共表达，随后在发育中的心脏瓣膜中特异性表达。所有这三个基因表现出不同的表达模式在呼吸和嗅觉上皮和/或间充质，并在发育中的牙齿。只有Raldh1表达在出生后的特定大脑结构中可见。这些数据表明，在各种分化器官中需要调节RA合成。(C)2002爱思唯尔科学爱尔兰有限公司保留所有权利。
Three retinaldehyde dehydrogenases (RALDH1, RALDH2 and RALDH3), which catalyze the oxidation of retinaldehyde into retinoic acid, have been shown to be differentially expressed during early embryogenesis. Here, we report their differential expression patterns throughout later mouse organogenesis. Raldh1 is prominently expressed in developing lung (notably in bronchial and tracheal epithelia), and shows stage-specific expression in stomach and intestine epithelial and mesenchymal layers. Raldh3 expression is specific to the differentiating intestinal lamina propria. Raldh2 is expressed throughout the kidney nephrogenic zone, whereas Raldh1 and Raldh3 are mostly expressed in collecting duct epithelia. Raldh3 expression is more restricted than that of Raldh1 in the urogenital tract and sex gland epithelia, whereas Raldh2 expression is mesenchymal. Raldh1 is coexpressed with Raldh2 in the early heart epicardium, and is later specifically expressed in developing heart valves. All three genes exhibit distinct expression patterns in respiratory and olfactory epithelia and/or mesenchymes, and in developing teeth. Only Raldh1 expression is seen after birth in specific brain structures. These data indicate a requirement for regulated RA synthesis in various differentiating organs. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.