Biosynthesis of mono- and sesquiterpenes in carrot roots and leaves (Daucus carota L.):: metabolic cross talk of cytosolic mevalonate and plastidial methylerythritol phosphate pathways
Biosynthesis of mono- and sesquiterpenes in carrot roots and leaves (Daucus carota L.):: metabolic cross talk of cytosolic mevalonate and plastidial methylerythritol phosphate pathways
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DOI：
10.1016/j.phytochem.2004.12.010
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发表时间：
2005-02-01
期刊：
影响因子：
3.8
通讯作者：
Wüst, M
中科院分区：
文献类型：
--
作者：
Hampel, D;Mosandl, A;Wüst, M
以胡萝卜（Daucus carota L.）通过用[5，5-H-2（2）]-甲羟戊酸内酯（d（2）-MVL）和[5，5-H-2（2）]-1-脱氧-D-木酮糖（d（2）-DOX）进行体内饲养实验来研究品种Bolero和Kazan）。采用搅拌棒吸附萃取法提取组织中的挥发性成分，采用热解吸-多维气相色谱-质谱联用技术进行分析。实验表明胡萝卜根和胡萝卜叶中萜类化合物的独立从头生物合成。在这两种植物组织中，单萜仅通过1-脱氧-D-木酮糖/2-C-甲基-D-β-4-磷酸（DOXP/MEP）途径生物合成，而倍半萜通过经典的甲羟戊酸途径以及DOXP/MEP途径产生。对胡萝卜根组织更详细的研究表明，萜烯类化合物的生物合成主要位于韧皮部。然而，在木质部的萜烯的从头生物合成是可检测的，即使在没有油导管在这个组织。(C)2004 Elsevier Ltd.保留所有权利。
The biosynthesis of the monoterpenes terpinolene and myrcene and the sesquiterpene beta-caryophyllene in roots and leaves of two carrot varieties (Daucus carota L. cultivars Bolero and Kazan) were investigated by in vivo feeding experiments with [5,5-H-2(2)]-mevalonic acid lactone (d(2)-MVL) and [5,5-H-2(2)]-1-deoxy-D-xylulose (d(2)-DOX). The volatiles of the tissues were extracted by stir bar sorptive extraction and analyzed using thermal desorption-multidimensional gas chromatography-mass spectrometry. The experiments demonstrate independent de novo-biosynthesis of terpenoids in carrot roots and in carrot leaves. In both plant tissues monoterpenes are biosynthesized exclusively via the 1-deoxy-D-xylulose/2-C-methyl-D-erythritol-4-phosphate (DOXP/MEP) pathway, whereas sesquiterpenes are generated by the classical mevalonic acid pathway as well as by the DOXP/MEP route. A more detailed investigation of carrot root tissues revealed that the biosynthesis of terpenes is mainly localized in the phloem. Nevertheless, in xylem a de novo-biosynthesis of terpenes was detectable as well, even in the absence of oil ducts in this tissue. (C) 2004 Elsevier Ltd. All rights reserved.