Class-Specific Evolution and Transcriptional Differentiation of 14-3-3 Family Members in Mesohexaploid Brassica rapa
Class-Specific Evolution and Transcriptional Differentiation of 14-3-3 Family Members in Mesohexaploid Brassica rapa
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DOI：
10.3389/fitte2016.00012
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发表时间：
2016-01-26
影响因子：
5.6
通讯作者：
Bisht, Naveen C.
中科院分区：
文献类型：
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作者：
Chandna, Ruby;Augustine, Rehna;Bisht, Naveen C.
14-3-3s是高度保守的多基因家族蛋白，其参与调节各种生物学过程。固有的多倍性和基因组复杂性的存在限制了从全球重要的芸苔属作物中鉴定和表征14-3-3蛋白。通过对模式芸苔属基因组芜菁（Brassica rapa）的数据挖掘，我们鉴定了编码14-3-3蛋白的21个成员，即BraA.GRF14.a至BraA.GRF14.u。系统发育分析表明，B. rapa含有E（epsilon）和非t14 -3-3亚型，具有不同的内含子-外显子结构组织模式。与E蛋白亚型（Ks > 0.48）相比，非t亚型显示出较低的分歧率（Ks <0.45），表明类别特异性分歧模式。同线分析表明，中六倍体B.芜菁基因组保留了每个拟南芥14-3-3基因的1-5个直系同源物，散布在其三个片段化的亚基因组中。qRT-PCR分析显示21个BraA.GRF14中的14个表达，其中与E基因相比，观察到更高丰度的非e转录物，表明类别特异性转录偏倚。BraA.GRF14基因在植物发育过程中以及在非生物胁迫、植物激素处理和营养剥夺条件下表现出不同的表达模式。总之，BraA. GRF 14基因的不同表达模式和差异调节表明B的功能分歧的发生。rapa 14-3-3蛋白在植物发育和胁迫反应中的作用
14-3-3s are highly conserved, multigene family proteins that have been implicated in modulating various biological processes. The presence of inherent polyploidy and genome complexity has limited the identification and characterization of 14-3-3 proteins from globally important Brassica crops. Through data mining of Brassica rapa, the model Brassica genome, we identified 21 members encoding 14-3-3 proteins namely, BraA.GRF14.a to BraA.GRF14.u. Phylogenetic analysis indicated that B. rapa contains both E, (epsilon) and non-t 14-3-3 isoforms, having distinct intron-exon structural organization patterns. The non-t isoforms showed lower divergence rate (Ks < 0.45) compared to E protein isoforms (Ks > 0.48), suggesting class-specific divergence pattern. Synteny analysis revealed that mesohexaploid B. rapa genome has retained 1-5 orthologs of each Arabidopsis 14-3-3 gene, interspersed across its three fragmented sub-genomes. qRT-PCR analysis showed that 14 of the 21 BraA.GRF14 were expressed, wherein a higher abundance of non-e transcripts was observed compared to the E genes, indicating class-specific transcriptional bias. The BraA.GRF14 genes showed distinct expression pattern during plant developmental stages and in response to abiotic stress, phytohormone treatments, and nutrient deprivation conditions. Together, the distinct expression pattern and differential regulation of BraA.GRF14 genes indicated the occurrence of functional divergence of B. rapa 14-3-3 proteins during plant development and stress responses.