Striatal dopamine release and metabolism in sinoaortic-denervated rats by in vivo microdialysis.
Striatal dopamine release and metabolism in sinoaortic-denervated rats by in vivo microdialysis.
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通过体内微透析观察去神经主动脉大鼠纹状体多巴胺的释放和代谢。
DOI：
10.1152/ajpregu.1988.254.2.r396
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
T. Nagatsu
中科院分区：
文献类型：
--
作者：
N. Alexander;D. Nakahara;N. Ozaki;N. Kaneda;T. Sasaoka;N. Iwata;T. Nagatsu
本研究的目的是提供支持这一假设的新证据，即来自动脉压力感受器的心血管信息与有助于调节运动活动的黑质纹状体系统相结合。采用体内微透析技术收集细胞外纹状体多巴胺（DA）及其代谢物二羟基苯乙酸（DOPAC）和高香草酸（HVA）样品，并通过高效液相色谱-电子捕获检测进行分析。将导管放置在大鼠尾壳核中，以便随后插入微透析探针。在去主动脉神经（SAD）或假手术（SO）后的第一周，在自由活动的大鼠中插入微透析探针并以2微升/分钟的速率灌注林格溶液。在腹腔注射 100 mg/kg 帕吉林之前和之后每 20 分钟收集一次样品。结果显示，SAD大鼠的细胞外纹状体DA、DOPAC和HVA比SO大鼠少约50%（P小于0.01）。用帕吉林阻断单胺氧化酶活性后，SO 中纹状体 DA 的积累速度比 SAD 大鼠快三倍，这表明 SAD 大鼠中 DA 合成减少。这些数据提供了进一步的证据，表明动脉压力感受器系统影响黑质纹状体系统中的多巴胺能代谢，可能作为整合心血管和运动活动的手段。
The purpose of this study was to provide new evidence favoring the hypothesis that cardiovascular information from arterial baroreceptors is integrated with the nigrostriatal system that contributes to regulation of motor activity. Samples of extracellular striatal dopamine (DA) and its metabolites, dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA), were collected by the technique of in vivo microdialysis and analyzed by high-performance liquid chromatography-electron capture detection. Rats were prepared with a guide tube placed in the caudate-putamen for subsequent insertion of microdialysis probes. During the 1st wk after sinoaortic denervation (SAD) or sham operation (SO), a microdialysis probe was inserted and perfused with Ringer solution at the rate of 2 microliter/min in the freely moving rats. Samples were collected every 20 min before and after injection of pargyline, 100 mg/kg ip. The results showed that SAD rats have approximately 50% less extracellular striatal DA, DOPAC, and HVA than SO rats (P less than 0.01). After blockade of monoamine oxidase activity with pargyline, striatal DA accumulated three times faster in SO than SAD rats suggesting DA synthesis is reduced in SAD rats. These data provide further evidence that the arterial baroreceptor system affects dopaminergic metabolism in the nigrostriatal system possibly as a means for integration of cardiovascular and motor activity.